53175

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 364395..364897 Replicon chromosome
Accession NZ_CP011018
Organism Escherichia coli strain CI5

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag VK74_RS01675 Protein ID WP_000767829.1
Coordinates 364643..364897 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag VK74_RS01670 Protein ID WP_001259255.1
Coordinates 364395..364646 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VK74_RS01650 359573..360640 - 1068 WP_000080114.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
VK74_RS01655 360640..361710 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
VK74_RS01660 361707..363011 - 1305 WP_021499265.1 histidinol dehydrogenase -
VK74_RS01665 363017..363916 - 900 WP_000131792.1 ATP phosphoribosyltransferase -
VK74_RS26100 364062..364112 - 51 WP_001364200.1 his operon leader peptide -
VK74_RS01670 364395..364646 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
VK74_RS01675 364643..364897 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
VK74_RS01680 364980..365804 + 825 WP_000754758.1 SDR family oxidoreductase -
VK74_RS01685 365850..366779 + 930 WP_046201426.1 LysR family transcriptional regulator -
VK74_RS28225 366994..367056 + 63 WP_010723108.1 membrane protein YoeI -
VK74_RS01690 367046..368404 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
VK74_RS01695 368583..369641 + 1059 WP_000492346.1 transport protein YeeE -
VK74_RS01700 369655..369882 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T53175 WP_000767829.1 NZ_CP011018:364643-364897 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T53175 NZ_CP011018:364643-364897 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT53175 WP_001259255.1 NZ_CP011018:364395-364646 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT53175 NZ_CP011018:364395-364646 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References