Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 364395..364897 Replicon chromosome
Accession NZ_CP011018
Organism Escherichia coli strain CI5

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag VK74_RS01675 Protein ID WP_000767829.1
Coordinates 364643..364897 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag VK74_RS01670 Protein ID WP_001259255.1
Coordinates 364395..364646 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VK74_RS01650 359573..360640 - 1068 WP_000080114.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
VK74_RS01655 360640..361710 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
VK74_RS01660 361707..363011 - 1305 WP_021499265.1 histidinol dehydrogenase -
VK74_RS01665 363017..363916 - 900 WP_000131792.1 ATP phosphoribosyltransferase -
VK74_RS26100 364062..364112 - 51 WP_001364200.1 his operon leader peptide -
VK74_RS01670 364395..364646 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
VK74_RS01675 364643..364897 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
VK74_RS01680 364980..365804 + 825 WP_000754758.1 SDR family oxidoreductase -
VK74_RS01685 365850..366779 + 930 WP_046201426.1 LysR family transcriptional regulator -
VK74_RS28225 366994..367056 + 63 WP_010723108.1 membrane protein YoeI -
VK74_RS01690 367046..368404 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
VK74_RS01695 368583..369641 + 1059 WP_000492346.1 transport protein YeeE -
VK74_RS01700 369655..369882 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T53175 WP_000767829.1 NZ_CP011018:364643-364897 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T53175 NZ_CP011018:364643-364897 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT53175 WP_001259255.1 NZ_CP011018:364395-364646 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT53175 NZ_CP011018:364395-364646 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References