Detailed information of TA system TA053151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 56698..57112
• Replicon: chromosome
• Accession: NZ_CP010998
• Organism: Staphylococcus aureus strain FORC_012 isolate human sputum

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FORC12_RS00385
• Protein ID: WP_061477612.1
• Coordinates: 56698..56949 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FORC12_RS15180
• Protein ID: WP_000028424.1
• Coordinates: 56939..57112 (+)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC12_RS00335	52037..52708	+	672	WP_016028251.1	hypothetical protein	-
FORC12_RS00340	52818..53477	-	660	WP_016028252.1	hypothetical protein	-
FORC12_RS00345	53523..54293	+	771	WP_000190226.1	conserved phage C-terminal domain-containing protein	-
FORC12_RS00350	54303..55076	+	774	WP_000803028.1	ATP-binding protein	-
FORC12_RS00355	55070..55228	+	159	WP_000256589.1	hypothetical protein	-
FORC12_RS00360	55242..55463	+	222	WP_001123681.1	DUF3269 family protein	-
FORC12_RS00365	55474..55878	+	405	WP_000049798.1	DUF1064 domain-containing protein	-
FORC12_RS00370	55883..56068	+	186	WP_016028253.1	DUF3113 family protein	-
FORC12_RS00375	56069..56437	+	369	WP_016028254.1	hypothetical protein	-
FORC12_RS00380	56441..56683	+	243	WP_000131389.1	hypothetical protein	-
FORC12_RS00385	56698..56949	+	252	WP_061477612.1	DUF1024 family protein	Toxin
FORC12_RS15180	56939..57112	+	174	WP_000028424.1	hypothetical protein	Antitoxin
FORC12_RS00390	57113..57394	+	282	WP_000454994.1	hypothetical protein	-
FORC12_RS15185	57395..57556	+	162	WP_000889683.1	hypothetical protein	-
FORC12_RS00395	57571..58104	+	534	WP_016028255.1	dUTP diphosphatase	-
FORC12_RS00400	58141..58347	+	207	WP_016028256.1	DUF1381 domain-containing protein	-
FORC12_RS00405	58344..58517	+	174	WP_000595257.1	transcriptional activator RinB	-
FORC12_RS00410	58518..58880	+	363	WP_000383791.1	hypothetical protein	-
FORC12_RS00415	58881..59027	+	147	WP_000989961.1	hypothetical protein	-
FORC12_RS00420	59042..59461	+	420	WP_000058634.1	transcriptional regulator	-
FORC12_RS00425	59648..60088	+	441	WP_009560332.1	terminase small subunit	-
FORC12_RS00430	60075..61352	+	1278	WP_009560330.1	PBSX family phage terminase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	44581..86761	42180

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9361.22 Da
• Isoelectric Point: 3.8464

>T53151 WP_061477612.1 NZ_CP010998:56698-56949 [Staphylococcus aureus]
MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILDGMTNAIQHSVKEGVELDEAVGIMAGQVVYKYEEEQE
NEY

Nucleotide

• Length: 252 bp

>T53151 NZ_CP010998:56698-56949 [Staphylococcus aureus]
ATGAATAATCGTGAACAAATTGAACAATCCGTTATAAGTGCTAGTGCGTATAACGGTAATGATACAGAGGGATTACTAAA
AGAGATTGAAGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGATGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6468.29 Da
• Isoelectric Point: 4.2484

>AT53151 WP_000028424.1 NZ_CP010998:56939-57112 [Staphylococcus aureus]
MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIIDFITKPIYLIKGDE

Nucleotide

• Length: 174 bp

>AT53151 NZ_CP010998:56939-57112 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCCGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTGA