Detailed information of TA system TA053148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 167509..168188
• Replicon: plasmid pFRP1-1
• Accession: NZ_CP010990
• Organism: Pseudonocardia sp. EC080625-04

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: FRP1_RS29405
• Protein ID: WP_060722538.1
• Coordinates: 167814..168188 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: -
• Locus tag: FRP1_RS29400
• Protein ID: WP_060722537.1
• Coordinates: 167509..167817 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRP1_RS31275	162598..163468	-	871	Protein_151	IS630 family transposase	-
FRP1_RS32515	163975..164382	+	408	WP_168170589.1	integrase core domain-containing protein	-
FRP1_RS31945	164745..165128	+	384	WP_145982881.1	transposase	-
FRP1_RS29390	165452..165868	+	417	Protein_154	transposase	-
FRP1_RS29395	166207..167405	+	1199	Protein_155	IS110 family transposase	-
FRP1_RS29400	167509..167817	-	309	WP_060722537.1	helix-turn-helix transcriptional regulator	Antitoxin
FRP1_RS29405	167814..168188	-	375	WP_060722538.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FRP1_RS29410	168271..169362	-	1092	WP_060575005.1	IS630 family transposase	-
FRP1_RS29415	169517..170806	+	1290	Protein_159	IS701 family transposase	-
FRP1_RS29420	171144..171458	+	315	WP_060577632.1	multidrug efflux SMR transporter	-
FRP1_RS29425	171458..171835	+	378	WP_060577633.1	multidrug efflux SMR transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..297301	297301

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14151.85 Da
• Isoelectric Point: 4.6830

>T53148 WP_060722538.1 NZ_CP010990:c168188-167814 [Pseudonocardia sp. EC080625-04]
MWEIYLTDEVHEWLDELLRGDENTHYQVVSAIEALAEGGPNLGRPLVDRIKGSAVYNLKELRPGSAGSSEVRILFAFDPW
RSSVLLVAGDKAGRWTEWYRQAIPRAEQLYETYVKERAAEEGRS

Nucleotide

• Length: 375 bp

>T53148 NZ_CP010990:c168188-167814 [Pseudonocardia sp. EC080625-04]
ATGTGGGAGATCTACCTGACCGACGAGGTGCACGAGTGGCTCGACGAGCTGCTCCGCGGCGACGAGAACACCCACTACCA
GGTGGTCTCGGCGATCGAGGCTCTCGCGGAGGGTGGGCCGAACCTGGGGCGTCCGCTGGTAGACCGGATCAAGGGCTCGG
CGGTCTACAACTTGAAGGAGCTGCGTCCGGGGTCAGCCGGGAGCAGCGAGGTGCGGATCTTGTTTGCGTTCGATCCGTGG
CGTAGCTCGGTGCTGCTGGTCGCGGGGGACAAGGCCGGGCGCTGGACGGAGTGGTATCGGCAGGCCATCCCGCGGGCTGA
GCAGCTCTACGAGACGTACGTGAAGGAACGCGCAGCCGAGGAGGGCAGGTCATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10970.50 Da
• Isoelectric Point: 11.3231

>AT53148 WP_060722537.1 NZ_CP010990:c167817-167509 [Pseudonocardia sp. EC080625-04]
MSTVRWSEIRGEHVAAAGGGRVEQGKARLLGQVRAHRLAETRKRRGLTQRQVAQAMGVTVGRVSQIENGELAGIDVLDRY
VHALGGGLQIVANFGDEQIKVG

Nucleotide

• Length: 309 bp

>AT53148 NZ_CP010990:c167817-167509 [Pseudonocardia sp. EC080625-04]
ATGAGCACGGTTCGATGGTCGGAGATCCGGGGCGAGCACGTCGCGGCCGCGGGGGGGGGGCGCGTCGAGCAGGGCAAGGC
CCGGCTGCTCGGTCAGGTGCGGGCGCATCGGCTGGCGGAGACGCGCAAGCGTCGCGGGCTGACCCAGCGCCAGGTGGCAC
AGGCGATGGGCGTGACCGTCGGGCGGGTCAGCCAGATTGAGAACGGCGAGCTGGCCGGGATCGACGTGCTCGACCGCTAC
GTGCACGCGCTGGGCGGGGGCTTGCAGATCGTGGCGAACTTCGGGGACGAGCAGATCAAGGTAGGCTGA