Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 6026534..6027324 Replicon chromosome
Accession NZ_CP010979
Organism Pseudomonas putida S13.1.2

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0C5S5K2
Locus tag N805_RS26635 Protein ID WP_019473276.1
Coordinates 6026534..6027001 (-) Length 156 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID I7C311
Locus tag N805_RS26640 Protein ID WP_014859917.1
Coordinates 6026998..6027324 (-) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N805_RS26615 6021697..6023226 + 1530 WP_019473279.1 efflux transporter outer membrane subunit -
N805_RS26620 6023223..6025400 + 2178 WP_019473278.1 FUSC family protein -
N805_RS26625 6025390..6025590 + 201 WP_009685040.1 DUF1656 domain-containing protein -
N805_RS26630 6025601..6026461 + 861 WP_019473277.1 HlyD family secretion protein -
N805_RS26635 6026534..6027001 - 468 WP_019473276.1 type II toxin-antitoxin system YhaV family toxin Toxin
N805_RS26640 6026998..6027324 - 327 WP_014859917.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
N805_RS26645 6027417..6027863 - 447 WP_019473275.1 universal stress protein -
N805_RS26650 6028134..6029039 + 906 WP_016497993.1 LysR family transcriptional regulator -
N805_RS26655 6029156..6030697 + 1542 WP_019473274.1 MFS transporter -
N805_RS26660 6030726..6031787 + 1062 WP_019473273.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(17-152)

Antitoxin

PrlF antitoxin

(8-106)


Sequences

Toxin        


Download         Length: 156 a.a.        Molecular weight: 17993.40 Da        Isoelectric Point: 9.9698

>T53145 WP_019473276.1 NZ_CP010979:c6027001-6026534 [Pseudomonas putida S13.1.2]
MSDTSRKPLVIHGWTVIAHPLFLAKLDALSAQVEAQRHKDPTGYTKRNAFKRLAAIRRLAFDVIPQDPTKPEYRQGATLG
GDHKHWFRAKFFQQYRLFFRYHTPSRIIVLAWVNDESSKRAYESSDDAYKVFQKMLHSGHPPDDWDQLLQEAAAD

Download         Length: 468 bp

>T53145 NZ_CP010979:c6027001-6026534 [Pseudomonas putida S13.1.2]
ATGAGTGATACGAGCAGGAAGCCTCTGGTTATTCATGGATGGACTGTAATTGCCCACCCGCTGTTCCTGGCCAAACTGGA
CGCGCTAAGCGCTCAAGTCGAGGCTCAACGGCACAAAGACCCAACGGGTTATACGAAAAGAAATGCCTTTAAAAGGCTGG
CCGCCATCCGGCGGTTGGCGTTCGATGTAATCCCTCAAGACCCGACCAAACCCGAATACCGGCAAGGGGCAACACTTGGC
GGTGACCACAAGCACTGGTTCAGAGCAAAGTTCTTCCAGCAGTACCGGTTATTCTTCCGCTACCACACCCCCAGCAGGAT
CATCGTGCTGGCCTGGGTCAATGACGAGTCGAGCAAGCGGGCCTACGAAAGTAGCGACGATGCTTACAAGGTCTTTCAAA
AGATGTTGCATAGCGGCCACCCACCGGACGATTGGGACCAGTTGCTGCAAGAGGCTGCCGCAGATTGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 11919.40 Da        Isoelectric Point: 4.3722

>AT53145 WP_014859917.1 NZ_CP010979:c6027324-6026998 [Pseudomonas putida S13.1.2]
MAATFDVESTLTDRYQTTVPETVRRALGLKKRDKIHYSIRPDGGVILTRAEATEDDPVLGKFLDFLAHDIATNPQRLQVV
DAGLIARLDKLVGDAEVDLDQPLSADDE

Download         Length: 327 bp

>AT53145 NZ_CP010979:c6027324-6026998 [Pseudomonas putida S13.1.2]
ATGGCTGCCACCTTCGACGTCGAGTCCACGCTGACCGATCGCTATCAAACCACTGTTCCCGAAACGGTGCGGCGCGCCTT
GGGCTTGAAAAAACGTGACAAGATCCATTACTCGATTCGCCCCGACGGCGGGGTGATACTCACCCGCGCAGAGGCAACCG
AGGACGATCCGGTACTGGGCAAATTTCTGGATTTCCTGGCACACGATATCGCTACCAACCCTCAACGGCTGCAGGTTGTC
GATGCTGGCCTCATCGCGCGCCTCGACAAGCTTGTCGGCGACGCGGAGGTCGACCTGGATCAACCTCTGTCGGCGGACGA
TGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C5S5K2

Antitoxin

Source ID Structure
AlphaFold DB I7C311

References