Detailed information of TA system TA053142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4273802..4274411
• Replicon: chromosome
• Accession: NZ_CP010979
• Organism: Pseudomonas putida S13.1.2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0C5S148
• Locus tag: N805_RS18870
• Protein ID: WP_026034476.1
• Coordinates: 4273802..4274098 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: N805_RS18875
• Protein ID: WP_019471683.1
• Coordinates: 4274100..4274411 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N805_RS18855	4269335..4269874	+	540	WP_019471679.1	sigma-70 family RNA polymerase sigma factor	-
N805_RS18860	4269867..4270835	+	969	WP_019471680.1	FecR domain-containing protein	-
N805_RS18865	4270915..4273344	+	2430	WP_033692291.1	TonB-dependent siderophore receptor	-
N805_RS18870	4273802..4274098	+	297	WP_026034476.1	DNA-binding protein	Toxin
N805_RS18875	4274100..4274411	+	312	WP_019471683.1	helix-turn-helix domain-containing protein	Antitoxin
N805_RS18880	4274473..4275402	-	930	WP_026034477.1	malate dehydrogenase	-
N805_RS18885	4275577..4275921	-	345	WP_019471685.1	type II toxin-antitoxin system RelE/ParE family toxin	-
N805_RS30065	4275935..4276183	-	249	WP_080956824.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
N805_RS18890	4276585..4276998	-	414	WP_019471687.1	hypothetical protein	-
N805_RS18895	4277052..4277294	-	243	WP_019471688.1	hypothetical protein	-
N805_RS18900	4277719..4277946	-	228	WP_028614148.1	hypothetical protein	-
N805_RS18905	4278565..4278792	-	228	WP_019471225.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-87)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10949.57 Da
• Isoelectric Point: 8.0083

>T53142 WP_026034476.1 NZ_CP010979:4273802-4274098 [Pseudomonas putida S13.1.2]
MRTVIETPTFQKAAARLWSEEERVAFIDWIAINPLAGDVIPGADGARKIRWNRSGCGKSSGTRVIYFNLHEDEIVLLLTV
YTKAERSNMSPADISKVV

Nucleotide

• Length: 297 bp

>T53142 NZ_CP010979:4273802-4274098 [Pseudomonas putida S13.1.2]
ATGCGCACAGTAATTGAGACGCCCACGTTTCAGAAAGCGGCAGCCAGGTTGTGGTCTGAAGAAGAGCGCGTAGCTTTCAT
CGACTGGATTGCGATCAACCCGCTTGCAGGAGATGTCATACCCGGTGCAGATGGCGCGAGAAAAATACGTTGGAATCGCT
CTGGCTGCGGTAAATCCAGCGGTACTCGAGTGATTTACTTCAACCTGCACGAAGATGAAATTGTGCTGTTGCTAACTGTT
TATACCAAGGCCGAGCGCAGCAACATGAGCCCTGCCGACATCAGCAAGGTGGTATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11325.12 Da
• Isoelectric Point: 5.8848

>AT53142 WP_019471683.1 NZ_CP010979:4274100-4274411 [Pseudomonas putida S13.1.2]
MRDIESIAKAIEADAGEPLPDLRQALKEAKVGAGRVTTPEQILVRQARERCGLTQASFAERIATPVATLRDWEQGRFVPP
GGVLCLLRLILKHPELSQELAEI

Nucleotide

• Length: 312 bp

>AT53142 NZ_CP010979:4274100-4274411 [Pseudomonas putida S13.1.2]
ATGCGTGATATTGAAAGTATTGCCAAGGCCATCGAGGCCGATGCAGGTGAACCGCTCCCTGACCTGCGTCAAGCACTCAA
GGAAGCCAAGGTGGGCGCCGGACGGGTAACCACCCCAGAGCAGATTCTGGTGCGCCAGGCCCGCGAGCGATGCGGCCTGA
CCCAGGCTTCTTTTGCCGAGCGCATCGCAACGCCAGTGGCCACCCTGCGGGATTGGGAACAGGGCCGCTTCGTGCCTCCG
GGTGGTGTATTGTGCCTTTTGCGCCTGATCCTCAAGCACCCTGAGCTATCACAGGAACTGGCCGAGATCTGA

Structures

Toxin

• AlphaFold DB: A0A0C5S148