Detailed information of TA system TA053140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3774526..3775112
• Replicon: chromosome
• Accession: NZ_CP010979
• Organism: Pseudomonas putida S13.1.2

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: N805_RS30000
• Protein ID: WP_080956820.1
• Coordinates: 3774526..3774804 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: N805_RS16665
• Protein ID: WP_003252274.1
• Coordinates: 3774813..3775112 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N805_RS16655	3771290..3772486	+	1197	WP_019472226.1	succinyldiaminopimelate transaminase	-
N805_RS16660	3772628..3774274	-	1647	WP_019472227.1	Na+/H+ antiporter	-
N805_RS30000	3774526..3774804	+	279	WP_080956820.1	type II toxin-antitoxin system toxin GraT	Toxin
N805_RS16665	3774813..3775112	+	300	WP_003252274.1	type II toxin-antitoxin system antitoxin GraA	Antitoxin
N805_RS16670	3775237..3775584	+	348	WP_003252272.1	ArsC family reductase	-
N805_RS16675	3775616..3776650	+	1035	WP_019472228.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
N805_RS16680	3776771..3777976	+	1206	WP_019472229.1	cysteine desulfurase	-
N805_RS16685	3777973..3778383	+	411	WP_019472230.1	SufE family protein	-
N805_RS16690	3778493..3779302	-	810	WP_177313747.1	tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-90)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10503.82 Da
• Isoelectric Point: 7.8929

>T53140 WP_080956820.1 NZ_CP010979:3774526-3774804 [Pseudomonas putida S13.1.2]
MIRSFACADTEALFTTGKTRRWSDIKSVAERKLAMLDAASELRDLRSPPGNRLESLSGNRSGQHSIRVNDQWRLCFTWTE
HGPVNVEIVDYH

Nucleotide

• Length: 279 bp

>T53140 NZ_CP010979:3774526-3774804 [Pseudomonas putida S13.1.2]
ATGATTCGAAGCTTTGCCTGTGCCGACACGGAAGCACTCTTCACCACTGGAAAGACGCGGCGTTGGTCAGATATCAAGTC
AGTCGCGGAGCGCAAACTGGCCATGCTCGATGCCGCCTCAGAGTTGCGAGATCTTCGGTCGCCACCTGGAAATCGCCTCG
AGTCACTGAGCGGCAACCGTTCCGGTCAACATAGTATCCGTGTGAATGACCAATGGCGCCTGTGCTTCACCTGGACCGAA
CACGGGCCAGTTAACGTTGAAATTGTCGATTACCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10891.52 Da
• Isoelectric Point: 5.6714

>AT53140 WP_003252274.1 NZ_CP010979:3774813-3775112 [Pseudomonas putida S13.1.2]
MLKNGMRPIHPGEILREDFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA

Nucleotide

• Length: 300 bp

>AT53140 NZ_CP010979:3774813-3775112 [Pseudomonas putida S13.1.2]
ATGCTCAAGAACGGTATGCGTCCAATCCATCCCGGTGAAATCCTGCGTGAGGATTTCCAGAAGGAAATGGGCTTCAGTGC
TGCGGCACTGGCCAGAGCGTTGGGCGTTGCAACGCCAACCGTCAACAACATTCTGCGCGAAAGGGGCGGTGTTTCCGCCG
ACATGGCACTGCGCCTGTCCATCTGCCTGGACACCACGCCCGAGTTCTGGCTTAACCTGCAGACGGCCTTCGATTTGCGC
ACAGCCGAACAGCAACACGGCGATGAGATCATCGGGTCGGTGCAGCGCCTGGTCGCTTGA