Detailed information of TA system TA053108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 2281290..2281945
• Replicon: chromosome
• Accession: NZ_CP010945
• Organism: Pseudomonas fluorescens NCIMB 11764

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0K1QZB5
• Locus tag: B723_RS10315
• Protein ID: WP_031319086.1
• Coordinates: 2281290..2281634 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B723_RS10320
• Protein ID: WP_017340370.1
• Coordinates: 2281631..2281945 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B723_RS10290	2276294..2277238	+	945	WP_017340376.1	ubiquinol oxidase subunit II	-
B723_RS10295	2277242..2279272	+	2031	WP_017340375.1	cytochrome o ubiquinol oxidase subunit I	-
B723_RS10300	2279276..2279902	+	627	WP_017340374.1	cytochrome o ubiquinol oxidase subunit III	-
B723_RS10305	2279903..2280238	+	336	WP_017340373.1	cytochrome o ubiquinol oxidase subunit IV	-
B723_RS10310	2280250..2281137	+	888	WP_017340372.1	heme o synthase	-
B723_RS10315	2281290..2281634	+	345	WP_031319086.1	toxin	Toxin
B723_RS10320	2281631..2281945	+	315	WP_017340370.1	helix-turn-helix domain-containing protein	Antitoxin
B723_RS10325	2282006..2282206	-	201	Protein_2039	DUF3077 domain-containing protein	-
B723_RS10330	2282595..2284013	-	1419	WP_017340369.1	PLP-dependent aminotransferase family protein	-
B723_RS10335	2284162..2285262	+	1101	WP_017340368.1	hypothetical protein	-
B723_RS10340	2285305..2286585	+	1281	WP_017340367.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (42-94)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13140.94 Da
• Isoelectric Point: 9.9573

>T53108 WP_031319086.1 NZ_CP010945:2281290-2281634 [Pseudomonas fluorescens NCIMB 11764]
VRTVFFETSSFTATVGNYLTDDAYRALQTHMQLNPQAGDVMPRTGGFRKLRWADGRRGKGRRGGLRVIYYWLMNDGQFWM
FAIYDKDELENLSADQEKALKKAIETELKVRGTP

Nucleotide

• Length: 345 bp

>T53108 NZ_CP010945:2281290-2281634 [Pseudomonas fluorescens NCIMB 11764]
GTGCGAACCGTATTCTTCGAAACCTCTTCCTTCACTGCCACCGTTGGCAATTATCTGACGGACGATGCGTATCGTGCGTT
GCAAACCCATATGCAGCTGAATCCGCAGGCCGGCGACGTCATGCCTCGAACAGGCGGCTTCCGAAAGTTGCGCTGGGCAG
ATGGCCGTCGAGGTAAAGGCAGGCGAGGTGGCTTGCGGGTTATCTATTATTGGCTTATGAATGATGGCCAGTTCTGGATG
TTTGCAATTTATGACAAAGATGAGCTTGAGAATTTGAGCGCCGACCAAGAGAAAGCGCTCAAGAAAGCCATAGAGACAGA
GTTGAAAGTACGGGGTACCCCATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11800.70 Da
• Isoelectric Point: 10.0071

>AT53108 WP_017340370.1 NZ_CP010945:2281631-2281945 [Pseudomonas fluorescens NCIMB 11764]
MKKRDLFAELMQGVDEMAAQREGKITLRNTSIEDIPAPEVGAQEIVALREKLHMSQAVFAKRIRTSPSTLRNWEQEKSRP
NAQAALLIRLVEKFPDMVDRLAAV

Nucleotide

• Length: 315 bp

>AT53108 NZ_CP010945:2281631-2281945 [Pseudomonas fluorescens NCIMB 11764]
ATGAAAAAGCGCGACCTGTTTGCAGAATTGATGCAGGGCGTTGATGAGATGGCGGCTCAACGCGAAGGCAAGATCACGTT
GCGCAATACGTCAATTGAGGACATCCCTGCGCCTGAGGTAGGGGCGCAAGAGATCGTTGCACTGCGAGAAAAGTTACATA
TGTCTCAAGCCGTTTTTGCCAAGCGTATTCGAACCAGTCCGAGCACCCTGAGGAATTGGGAGCAGGAAAAATCGAGACCT
AATGCCCAAGCTGCCTTGCTGATCAGGCTGGTGGAGAAATTCCCTGACATGGTTGATCGACTGGCAGCTGTTTGA

Structures

Toxin

• AlphaFold DB: A0A0K1QZB5