Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 456291..456954 Replicon chromosome
Accession NZ_CP010945
Organism Pseudomonas fluorescens NCIMB 11764

Toxin (Protein)


Gene name higB Uniprot ID A0A0K1QHM1
Locus tag B723_RS02190 Protein ID WP_017341131.1
Coordinates 456589..456954 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0K1QII6
Locus tag B723_RS02185 Protein ID WP_017341130.1
Coordinates 456291..456596 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B723_RS02175 452373..456119 + 3747 WP_017341129.1 bifunctional diguanylate cyclase/phosphodiesterase -
B723_RS02185 456291..456596 - 306 WP_017341130.1 XRE family transcriptional regulator Antitoxin
B723_RS02190 456589..456954 - 366 WP_017341131.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B723_RS02195 457448..457756 + 309 WP_017341132.1 DUF3077 domain-containing protein -
B723_RS02200 457822..458460 - 639 WP_031319159.1 peptidase M41 -
B723_RS02205 458700..459161 + 462 WP_031319160.1 Lrp/AsnC family transcriptional regulator -
B723_RS02210 459422..461104 + 1683 WP_017341135.1 NAD(P)/FAD-dependent oxidoreductase -
B723_RS02215 461117..461911 + 795 WP_017341136.1 carbon-nitrogen hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(14-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(14-86)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 14137.74 Da        Isoelectric Point: 5.2129

>T53105 WP_017341131.1 NZ_CP010945:c456954-456589 [Pseudomonas fluorescens NCIMB 11764]
MGWEVEYTDEFEGWWDRLNEAEQDSVQATVMLLGYEGPHLGFPYTSDIKGSRHGNLRELRVQHAGRPYRVLYAFDPRRCA
ILLIGGDKTGHDRWYLKHVPLAERLYDEHLETLKNEGYDNG

Download         Length: 366 bp

>T53105 NZ_CP010945:c456954-456589 [Pseudomonas fluorescens NCIMB 11764]
ATGGGTTGGGAAGTGGAATACACCGATGAATTTGAAGGCTGGTGGGATCGCCTCAACGAAGCAGAGCAGGACTCCGTACA
GGCAACAGTGATGTTGCTAGGTTATGAGGGACCGCACTTGGGTTTTCCTTACACCAGCGATATCAAGGGCTCCCGGCATG
GCAACCTCCGAGAGTTAAGGGTGCAACATGCTGGCAGACCCTACCGTGTGCTGTATGCCTTTGATCCTCGGAGATGCGCG
ATCCTGTTGATCGGCGGTGATAAAACCGGCCACGATCGTTGGTATCTGAAGCACGTGCCGCTAGCCGAACGTCTGTACGA
CGAGCATTTGGAAACTTTGAAGAACGAGGGCTATGACAATGGCTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11489.25 Da        Isoelectric Point: 9.7299

>AT53105 WP_017341130.1 NZ_CP010945:c456596-456291 [Pseudomonas fluorescens NCIMB 11764]
MAKKFADLVAGRSPETQDRVDALVRKMRAEMPLHELRQAQALSQETLAKTLHVNQAAISRMERRTDMYISTLRNYIRAMG
GELEIIATFPEGQVKIENFAN

Download         Length: 306 bp

>AT53105 NZ_CP010945:c456596-456291 [Pseudomonas fluorescens NCIMB 11764]
ATGGCTAAAAAATTCGCTGATTTAGTGGCGGGGCGCTCGCCTGAGACTCAGGATCGTGTCGACGCGCTGGTTCGAAAGAT
GCGAGCGGAAATGCCGTTGCACGAGTTGCGACAGGCTCAGGCATTGAGCCAGGAAACGTTGGCCAAAACCCTGCATGTCA
ATCAGGCGGCGATTTCGAGGATGGAGCGCCGCACTGACATGTACATCAGCACGTTGCGCAACTACATACGCGCAATGGGC
GGCGAACTGGAAATCATCGCGACCTTTCCTGAGGGTCAGGTAAAGATCGAAAACTTTGCAAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K1QHM1

Antitoxin

Source ID Structure
AlphaFold DB A0A0K1QII6

References