Detailed information of TA system TA053067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 3656140..3656794
• Replicon: chromosome
• Accession: NZ_CP010896
• Organism: Pseudomonas simiae strain PCL1751

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PF1751_RS16340
• Protein ID: WP_032883041.1
• Coordinates: 3656140..3656490 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: C3JY95
• Locus tag: PF1751_RS16345
• Protein ID: WP_010209571.1
• Coordinates: 3656480..3656794 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PF1751_RS16330	3652898..3654430	+	1533	WP_010209575.1	NADH-quinone oxidoreductase subunit M	-
PF1751_RS16335	3654438..3655901	+	1464	WP_010209574.1	NADH-quinone oxidoreductase subunit NuoN	-
PF1751_RS16340	3656140..3656490	+	351	WP_032883041.1	toxin	Toxin
PF1751_RS16345	3656480..3656794	+	315	WP_010209571.1	XRE family transcriptional regulator	Antitoxin
PF1751_RS16350	3656856..3657608	-	753	WP_010209570.1	outer membrane beta-barrel protein	-
PF1751_RS16355	3657742..3658569	-	828	WP_010209568.1	arylamine N-acetyltransferase	-
PF1751_RS16360	3658690..3658926	+	237	WP_010209566.1	hypothetical protein	-
PF1751_RS16365	3658958..3659158	-	201	WP_010209565.1	hypothetical protein	-
PF1751_RS29285	3659173..3659349	-	177	WP_010209563.1	hypothetical protein	-
PF1751_RS16370	3659463..3659984	+	522	WP_010209560.1	DUF3087 domain-containing protein	-
PF1751_RS16375	3660026..3661156	-	1131	WP_021492768.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13612.44 Da
• Isoelectric Point: 9.4016

>T53067 WP_032883041.1 NZ_CP010896:3656140-3656490 [Pseudomonas simiae]
MDALFIELPAFERHRKDYLSDELFHGFQQALMKNPEAGDVIEGTGGLRKVRFVDERRNKGQRGGLRVIYYGWSGGTQFWL
FTLYGKHEQDDLTPQHKKALKHMLDREIKARTHHET

Nucleotide

• Length: 351 bp

>T53067 NZ_CP010896:3656140-3656490 [Pseudomonas simiae]
ATGGATGCTCTATTTATCGAACTGCCGGCCTTCGAGCGGCATCGCAAGGACTATCTGAGTGACGAGCTTTTCCACGGTTT
TCAACAGGCGTTGATGAAGAACCCGGAAGCGGGAGACGTGATCGAGGGAACAGGTGGGTTACGCAAGGTTCGCTTTGTCG
ACGAACGACGCAACAAAGGCCAGCGCGGTGGCCTGCGGGTCATTTACTACGGGTGGTCAGGCGGCACGCAATTCTGGTTA
TTCACCCTGTACGGCAAACACGAACAGGACGACCTGACGCCTCAGCACAAAAAAGCCCTAAAACACATGCTGGACAGGGA
AATCAAAGCGAGAACACATCATGAAACGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11741.51 Da
• Isoelectric Point: 10.4449

>AT53067 WP_010209571.1 NZ_CP010896:3656480-3656794 [Pseudomonas simiae]
MKREIFSELVEGFDALANERQGKVTLRTHKVHLNNLEPLTAEEVVAVRQQLNLSRAVFAMYLRTNTRTLENWEQGRAKPN
AQAATLIRLVARFPETVAQLAALG

Nucleotide

• Length: 315 bp

>AT53067 NZ_CP010896:3656480-3656794 [Pseudomonas simiae]
ATGAAACGTGAAATCTTTTCCGAACTGGTCGAAGGCTTCGACGCCCTGGCAAACGAACGCCAAGGCAAAGTCACCCTGCG
GACCCACAAGGTTCATCTCAACAACCTGGAGCCGCTCACCGCAGAGGAAGTGGTCGCCGTGCGTCAACAGCTCAACCTCT
CCCGTGCCGTCTTCGCCATGTACCTGCGCACCAACACGCGTACGCTGGAGAACTGGGAGCAAGGCCGGGCTAAACCAAAT
GCCCAGGCCGCCACCCTGATTCGCCTGGTGGCGCGTTTTCCTGAGACCGTGGCACAACTTGCTGCGTTGGGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2N5F9B6