Detailed information of TA system TA053064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4460252..4460906
• Replicon: chromosome
• Accession: NZ_CP010892
• Organism: Pseudomonas sp. MRSN12121

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0C5EC26
• Locus tag: TO66_RS20280
• Protein ID: WP_044463940.1
• Coordinates: 4460252..4460602 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: TO66_RS20285
• Protein ID: WP_044463941.1
• Coordinates: 4460592..4460906 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TO66_RS20270	4457007..4458539	+	1533	WP_044463938.1	NADH-quinone oxidoreductase subunit M	-
TO66_RS20275	4458547..4460010	+	1464	WP_044463939.1	NADH-quinone oxidoreductase subunit NuoN	-
TO66_RS20280	4460252..4460602	+	351	WP_044463940.1	hypothetical protein	Toxin
TO66_RS20285	4460592..4460906	+	315	WP_044463941.1	transcriptional regulator	Antitoxin
TO66_RS20290	4461045..4462787	-	1743	WP_044463942.1	ABC transporter substrate-binding protein	-
TO66_RS20295	4462838..4463110	-	273	WP_044463943.1	DUF2160 domain-containing protein	-
TO66_RS20300	4463121..4463921	-	801	WP_044463944.1	carbohydrate ABC transporter permease	-
TO66_RS20305	4463932..4464798	-	867	WP_009049796.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-100)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13651.69 Da
• Isoelectric Point: 8.5210

>T53064 WP_044463940.1 NZ_CP010892:4460252-4460602 [Pseudomonas sp. MRSN12121]
MDALFIELPPFQRHRQGLLDDELFHSLQLELLKNPQAGDLIEGTGGLRKLRFVDERRHKGKRSGIRVIYYCWCAGAQFWL
FTLYAKNQQDDLAPHQKKLLKHLLNQEIEARTQHET

Nucleotide

• Length: 351 bp

>T53064 NZ_CP010892:4460252-4460602 [Pseudomonas sp. MRSN12121]
ATGGACGCCCTTTTTATCGAACTGCCCCCGTTCCAGCGCCATCGCCAGGGCCTCCTGGACGATGAGCTGTTTCACAGCCT
GCAGCTGGAGTTGCTGAAGAACCCGCAGGCCGGCGACCTCATCGAAGGCACCGGCGGCTTGCGCAAGCTGCGCTTTGTCG
ATGAACGCCGGCACAAGGGCAAGCGCAGCGGGATCAGGGTGATTTATTACTGCTGGTGCGCGGGCGCTCAGTTCTGGCTC
TTTACCCTTTACGCGAAGAACCAGCAGGACGACCTGGCACCGCACCAGAAGAAACTGCTCAAGCACCTGCTGAACCAGGA
AATCGAGGCGAGGACCCAACATGAAACGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11922.82 Da
• Isoelectric Point: 10.5451

>AT53064 WP_044463941.1 NZ_CP010892:4460592-4460906 [Pseudomonas sp. MRSN12121]
MKRDIFSELVEGFDALAKEREGKITLRTHKVKLNALLPITAEEVLAVREQLNLSRSVFAMYLRTNTRTLENWEQGRAKPN
AQATTLIRLVQRFPDTVERLATLA

Nucleotide

• Length: 315 bp

>AT53064 NZ_CP010892:4460592-4460906 [Pseudomonas sp. MRSN12121]
ATGAAACGTGACATCTTTTCCGAACTGGTCGAAGGTTTCGACGCCCTGGCCAAGGAGCGCGAGGGCAAAATCACACTGCG
CACCCACAAGGTGAAGCTGAACGCCTTGCTGCCGATCACCGCCGAAGAAGTGCTGGCGGTGCGCGAACAACTGAACCTGT
CCCGCTCGGTGTTTGCCATGTACCTGCGCACCAACACCCGGACCCTGGAAAACTGGGAACAAGGGCGCGCAAAACCCAAC
GCTCAGGCCACGACCCTGATCCGCCTGGTACAGCGCTTTCCCGATACTGTGGAGCGGCTGGCGACACTTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0C5EC26