Detailed information of TA system TA053022

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 74308..74547
• Replicon: plasmid pMNCRE44_6
• Accession: NZ_CP010882
• Organism: Escherichia coli strain MNCRE44

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: SY51_RS26690
• Protein ID: WP_023144756.1
• Coordinates: 74308..74442 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	74487..74547 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SY51_RS26660	70317..70718	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
SY51_RS30785	70651..70908	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
SY51_RS26670	71001..71654	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
SY51_RS26675	72596..73453	-	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
SY51_RS26680	73446..73520	-	75	WP_031943482.1	RepA leader peptide Tap	-
SY51_RS31150	73517..73651	-	135	Protein_90	protein CopA/IncA	-
SY51_RS26685	73757..74011	-	255	WP_000083850.1	replication regulatory protein RepA	-
SY51_RS26690	74308..74442	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	74487..74547	+	61	NuclAT_2	-	Antitoxin
-	74487..74547	+	61	NuclAT_2	-	Antitoxin
-	74487..74547	+	61	NuclAT_2	-	Antitoxin
-	74487..74547	+	61	NuclAT_2	-	Antitoxin
SY51_RS30970	74514..74800	-	287	Protein_93	DUF2726 domain-containing protein	-
SY51_RS26700	74878..76158	-	1281	Protein_94	IS66-like element ISEc23 family transposase	-
SY51_RS26705	76217..76921	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
SY51_RS26710	76972..77568	-	597	Protein_96	hypothetical protein	-
SY51_RS26725	78324..79301	+	978	WP_000361610.1	RepB family plasmid replication initiator protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	senB	1..122966	122966

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T53022 WP_023144756.1 NZ_CP010882:c74442-74308 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T53022 NZ_CP010882:c74442-74308 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT53022 NZ_CP010882:74487-74547 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin