Detailed information of TA system TA053018

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29432..29674
• Replicon: plasmid pMNCRE44_6
• Accession: NZ_CP010882
• Organism: Escherichia coli strain MNCRE44

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: SY51_RS26420
• Protein ID: WP_001312861.1
• Coordinates: 29516..29674 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	29432..29472 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SY51_RS26390	24627..26585	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
SY51_RS26395	26640..27074	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
SY51_RS26400	27071..27790	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
-	27802..27988	+	187	NuclAT_0	-	-
-	27802..27988	+	187	NuclAT_0	-	-
-	27802..27988	+	187	NuclAT_0	-	-
-	27802..27988	+	187	NuclAT_0	-	-
SY51_RS29875	28036..29405	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	29432..29472	+	41	NuclAT_1	-	Antitoxin
-	29432..29472	+	41	NuclAT_1	-	Antitoxin
-	29432..29472	+	41	NuclAT_1	-	Antitoxin
-	29432..29472	+	41	NuclAT_1	-	Antitoxin
SY51_RS26420	29516..29674	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
SY51_RS31140	30117..30452	+	336	WP_013023876.1	hypothetical protein	-
SY51_RS31145	30365..30571	+	207	WP_000275859.1	hypothetical protein	-
SY51_RS26435	30596..30883	+	288	WP_000107535.1	hypothetical protein	-
SY51_RS26440	31002..31823	+	822	WP_001234469.1	DUF945 domain-containing protein	-
SY51_RS26445	32120..32722	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
SY51_RS26450	33053..33436	+	384	WP_001151566.1	relaxosome protein TraM	-
SY51_RS26455	33570..34247	+	678	WP_001348626.1	PAS domain-containing protein	-
SY51_RS26460	34335..34562	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	senB	1..122966	122966

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T53018 WP_001312861.1 NZ_CP010882:29516-29674 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T53018 NZ_CP010882:29516-29674 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT53018 NZ_CP010882:29432-29472 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin