Detailed information of TA system TA053008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3556728..3557527
• Replicon: chromosome
• Accession: NZ_CP010876
• Organism: Escherichia coli strain MNCRE44

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: SY51_RS18045
• Protein ID: WP_000347251.1
• Coordinates: 3557063..3557527 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: SY51_RS18040
• Protein ID: WP_001296435.1
• Coordinates: 3556728..3557063 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SY51_RS18025	3552513..3553283	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
SY51_RS18030	3553299..3554633	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
SY51_RS18035	3555008..3556579	+	1572	WP_001273763.1	galactarate dehydratase	-
SY51_RS18040	3556728..3557063	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
SY51_RS18045	3557063..3557527	+	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
SY51_RS18050	3557582..3558391	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
SY51_RS18055	3558640..3559920	+	1281	WP_000681950.1	tagatose-bisphosphate aldolase subunit KbaZ	-
SY51_RS18060	3559943..3560416	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
SY51_RS18065	3560427..3561206	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
SY51_RS18070	3561196..3562074	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
SY51_RS18075	3562092..3562526	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T53008 WP_000347251.1 NZ_CP010876:3557063-3557527 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T53008 NZ_CP010876:3557063-3557527 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT53008 WP_001296435.1 NZ_CP010876:3556728-3557063 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT53008 NZ_CP010876:3556728-3557063 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829CJ20

Antitoxin

• AlphaFold DB: S1PPV5