Detailed information of TA system TA052948

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 28141..28405
• Replicon: plasmid pFORC11.2
• Accession: NZ_CP010831
• Organism: Shigella sonnei strain FORC_011

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: FORC11_RS26625
• Protein ID: WP_001303307.1
• Coordinates: 28141..28293 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	28343..28405 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC11_RS26595 (23344)	23344..25512	+	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-
FORC11_RS26600 (25588)	25588..26202	+	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
FORC11_RS26605 (26300)	26300..26509	+	210	WP_001140545.1	hemolysin expression modulator Hha	-
FORC11_RS33740 (26718)	26718..26894	+	177	WP_001054900.1	hypothetical protein	-
- (27380)	27380..27431	+	52	NuclAT_1	-	-
- (27380)	27380..27431	+	52	NuclAT_1	-	-
- (27380)	27380..27431	+	52	NuclAT_1	-	-
- (27380)	27380..27431	+	52	NuclAT_1	-	-
FORC11_RS26620 (27818)	27818..28069	+	252	WP_001291965.1	hypothetical protein	-
FORC11_RS26625 (28141)	28141..28293	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
- (28343)	28343..28405	+	63	NuclAT_0	-	Antitoxin
- (28343)	28343..28405	+	63	NuclAT_0	-	Antitoxin
- (28343)	28343..28405	+	63	NuclAT_0	-	Antitoxin
- (28343)	28343..28405	+	63	NuclAT_0	-	Antitoxin
FORC11_RS26630 (28585)	28585..29793	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
FORC11_RS26635 (29812)	29812..30882	+	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
FORC11_RS26640 (30875)	30875..33166	+	2292	WP_094190802.1	F-type conjugative transfer protein TrbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..83905	83905

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T52948 WP_001303307.1 NZ_CP010831:c28293-28141 [Shigella sonnei]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T52948 NZ_CP010831:c28293-28141 [Shigella sonnei]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT52948 NZ_CP010831:28343-28405 [Shigella sonnei]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin