Detailed information of TA system TA052941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3005977..3006704
• Replicon: chromosome
• Accession: NZ_CP010829
• Organism: Shigella sonnei strain FORC_011

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: FORC11_RS15820
• Protein ID: WP_000547563.1
• Coordinates: 3006393..3006704 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FORC11_RS15815
• Protein ID: WP_000126296.1
• Coordinates: 3005977..3006396 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC11_RS15795 (3001028)	3001028..3001555	-	528	WP_001078766.1	electron transport protein HydN	-
FORC11_RS15800 (3001703)	3001703..3002713	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
FORC11_RS15805 (3002973)	3002973..3004430	+	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
FORC11_RS15810 (3004439)	3004439..3005863	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
FORC11_RS15815 (3005977)	3005977..3006396	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
FORC11_RS15820 (3006393)	3006393..3006704	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
FORC11_RS15825 (3006866)	3006866..3007639	-	774	WP_001026459.1	hypothetical protein	-
FORC11_RS15830 (3007664)	3007664..3008134	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
FORC11_RS15835 (3008127)	3008127..3008537	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
FORC11_RS15840 (3008534)	3008534..3009301	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
FORC11_RS15845 (3009301)	3009301..3009843	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
FORC11_RS15850 (3009853)	3009853..3011550	-	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T52941 WP_000547563.1 NZ_CP010829:c3006704-3006393 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T52941 NZ_CP010829:c3006704-3006393 [Shigella sonnei]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT52941 WP_000126296.1 NZ_CP010829:c3006396-3005977 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT52941 NZ_CP010829:c3006396-3005977 [Shigella sonnei]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22