Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 298206..298747 Replicon chromosome
Accession NZ_CP010829
Organism Shigella sonnei strain FORC_011

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag FORC11_RS01455 Protein ID WP_000615976.1
Coordinates 298206..298484 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FORC11_RS01460 Protein ID WP_000729704.1
Coordinates 298487..298747 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC11_RS01440 (295788) 295788..296366 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
FORC11_RS01445 (296572) 296572..297339 + 768 WP_000333380.1 class II glutamine amidotransferase -
FORC11_RS01450 (297310) 297310..298050 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
FORC11_RS01455 (298206) 298206..298484 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FORC11_RS01460 (298487) 298487..298747 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FORC11_RS33090 (298875) 298875..299039 - 165 WP_237158187.1 hypothetical protein -
FORC11_RS01465 (298963) 298963..299706 + 744 WP_237158188.1 C40 family peptidase -
FORC11_RS32575 (300747) 300747..300875 + 129 Protein_275 ISAs1 family transposase -
FORC11_RS01475 (300946) 300946..301443 + 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
FORC11_RS01480 (301566) 301566..303278 - 1713 Protein_277 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - vgrG/tssI / rhs/PAAR / gmhA/lpcA 280908..336692 55784


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T52928 WP_000615976.1 NZ_CP010829:c298484-298206 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T52928 NZ_CP010829:c298484-298206 [Shigella sonnei]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT52928 WP_000729704.1 NZ_CP010829:c298747-298487 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT52928 NZ_CP010829:c298747-298487 [Shigella sonnei]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCTGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References