Detailed information of TA system TA052926

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 978274..978850
• Replicon: chromosome
• Accession: NZ_CP010827
• Organism: Corynebacterium singulare strain IBS B52218

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B6EPQ4
• Locus tag: CSING_RS04560
• Protein ID: WP_042530097.1
• Coordinates: 978274..978555 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSING_RS04565
• Protein ID: WP_186302134.1
• Coordinates: 978542..978850 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSING_RS04550	975142..976974	+	1833	WP_042530093.1	methionine--tRNA ligase	-
CSING_RS04555	976980..978173	+	1194	WP_042530095.1	MFS transporter	-
CSING_RS04560	978274..978555	+	282	WP_042530097.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSING_RS04565	978542..978850	+	309	WP_186302134.1	HigA family addiction module antidote protein	Antitoxin
CSING_RS04570	978887..979390	-	504	WP_042530100.1	GNAT family N-acetyltransferase	-
CSING_RS13885	979400..979591	-	192	WP_042530102.1	hypothetical protein	-
CSING_RS04580	979616..980440	+	825	WP_042530105.1	TatD family hydrolase	-
CSING_RS04585	980674..981822	+	1149	WP_042530107.1	resuscitation-promoting factor	-
CSING_RS04590	981857..982699	+	843	WP_042530110.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
CSING_RS04595	982696..983643	+	948	WP_042530112.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (7-85)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10952.60 Da
• Isoelectric Point: 10.6086

>T52926 WP_042530097.1 NZ_CP010827:978274-978555 [Corynebacterium singulare]
VIQSFADKVTERLWNREKVPSIDPRIHTVALRKLRQLGYAQRLDELRIPPGNRLEALKGVRQGQYSIRINSQWRICFRWT
AAGPQEVEIVDYH

Nucleotide

• Length: 282 bp

>T52926 NZ_CP010827:978274-978555 [Corynebacterium singulare]
GTGATCCAGTCGTTTGCGGACAAGGTGACCGAGCGTTTATGGAATCGTGAGAAAGTACCTTCCATCGACCCGCGTATTCA
CACGGTTGCTCTCCGTAAGTTGAGGCAGCTGGGTTATGCCCAGAGGCTGGATGAGCTCCGAATACCTCCGGGAAACAGAC
TCGAAGCGTTGAAGGGTGTTCGGCAGGGTCAGTACAGCATTCGCATCAACAGTCAATGGCGGATCTGTTTCCGATGGACC
GCCGCAGGGCCACAGGAGGTTGAGATCGTTGACTACCACTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11360.15 Da
• Isoelectric Point: 5.1273

>AT52926 WP_186302134.1 NZ_CP010827:978542-978850 [Corynebacterium singulare]
MTTTEKLPPVHPGEILVEDFLKEMGITQHKLAVSIGVPPRRINEIVHGKRAITADTALRLAKFFGMTPQFWLGLQSHYDL
DVAEDKILSELEEIEPLTPASA

Nucleotide

• Length: 309 bp

>AT52926 NZ_CP010827:978542-978850 [Corynebacterium singulare]
TTGACTACCACTGAGAAGCTCCCCCCAGTGCATCCAGGTGAAATTCTTGTGGAGGATTTCCTTAAGGAGATGGGGATAAC
CCAACACAAGCTCGCCGTATCGATTGGAGTTCCGCCTCGTCGGATCAATGAAATTGTCCACGGCAAGCGGGCTATCACCG
CTGATACTGCACTGCGGTTGGCCAAGTTTTTCGGAATGACTCCGCAATTTTGGCTAGGTCTCCAGTCGCACTACGACTTG
GATGTCGCGGAAGATAAAATCCTCTCTGAGTTAGAGGAAATTGAGCCGCTCACACCGGCTTCAGCCTAA

Structures

Toxin

• AlphaFold DB: A0A0B6EPQ4