Detailed information of TA system TA052897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 256571..257265
• Replicon: chromosome
• Accession: NZ_CP010816
• Organism: Escherichia coli strain BL21 (TaKaRa)

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: SR36_RS01200
• Protein ID: WP_001263493.1
• Coordinates: 256867..257265 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: SR36_RS01195
• Protein ID: WP_000554757.1
• Coordinates: 256571..256864 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SR36_RS01175 (SR36_01175)	252211..252708	+	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-
SR36_RS01180 (SR36_01180)	252924..254636	-	1713	Protein_224	flagellar biosynthesis protein FlhA	-
SR36_RS01185 (SR36_01185)	254608..255393	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
SR36_RS01190 (SR36_01190)	255464..256519	+	1056	WP_001226164.1	DNA polymerase IV	-
SR36_RS01195 (SR36_01195)	256571..256864	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
SR36_RS01200 (SR36_01200)	256867..257265	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
SR36_RS01205 (SR36_01205)	257275..257727	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
SR36_RS01210 (SR36_01210)	257973..258176	+	204	Protein_230	RNA ligase RtcB family protein	-
SR36_RS01215 (SR36_01215)	258175..258696	+	522	Protein_231	peptide chain release factor H	-
SR36_RS01220 (SR36_01220)	258753..260210	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
SR36_RS01225 (SR36_01225)	260471..260929	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
SR36_RS01230 (SR36_01230)	261021..262265	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T52897 WP_001263493.1 NZ_CP010816:256867-257265 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T52897 NZ_CP010816:256867-257265 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT52897 WP_000554757.1 NZ_CP010816:256571-256864 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT52897 NZ_CP010816:256571-256864 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6