52896

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 250535..251076 Replicon chromosome
Accession NZ_CP010816
Organism Escherichia coli strain BL21 (TaKaRa)

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag SR36_RS01160 Protein ID WP_000615981.1
Coordinates 250535..250813 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag SR36_RS01165 Protein ID WP_000729704.1
Coordinates 250816..251076 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SR36_RS01145 (SR36_01145) 248117..248695 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
SR36_RS01150 (SR36_01150) 248901..249668 + 768 WP_000333379.1 class II glutamine amidotransferase -
SR36_RS01155 (SR36_01155) 249639..250379 - 741 WP_001225679.1 murein L,D-transpeptidase -
SR36_RS01160 (SR36_01160) 250535..250813 - 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
SR36_RS01165 (SR36_01165) 250816..251076 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
SR36_RS01170 (SR36_01170) 251262..252035 + 774 WP_001353765.1 C40 family peptidase -
SR36_RS01175 (SR36_01175) 252211..252708 + 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
SR36_RS01180 (SR36_01180) 252924..254636 - 1713 Protein_224 flagellar biosynthesis protein FlhA -
SR36_RS01185 (SR36_01185) 254608..255393 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T52896 WP_000615981.1 NZ_CP010816:c250813-250535 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T52896 NZ_CP010816:c250813-250535 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT52896 WP_000729704.1 NZ_CP010816:c251076-250816 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT52896 NZ_CP010816:c251076-250816 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References