Detailed information of TA system TA052810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5320698..5321293
• Replicon: chromosome
• Accession: NZ_CP010555
• Organism: Pseudomonas aeruginosa strain FRD1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: EG09_RS31930
• Protein ID: WP_003117425.1
• Coordinates: 5320698..5320976 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EG09_RS24315
• Protein ID: WP_003099268.1
• Coordinates: 5320988..5321293 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EG09_RS24300	5316128..5317366	+	1239	WP_014603324.1	C69 family dipeptidase	-
EG09_RS24305	5317428..5318075	+	648	WP_003095021.1	carbonate dehydratase	-
EG09_RS24310	5318145..5320373	-	2229	WP_003113525.1	TonB-dependent receptor	-
EG09_RS31930	5320698..5320976	+	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EG09_RS24315	5320988..5321293	+	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
EG09_RS24320	5321630..5322022	+	393	WP_025297673.1	hypothetical protein	-
EG09_RS32360	5322083..5323174	-	1092	WP_124135598.1	hypothetical protein	-
EG09_RS24325	5323227..5323562	-	336	WP_025297674.1	hypothetical protein	-
EG09_RS24330	5324009..5324281	+	273	WP_004352675.1	hypothetical protein	-
EG09_RS24335	5324457..5324747	+	291	WP_033896069.1	DUF5447 family protein	-
EG09_RS24345	5325263..5325697	+	435	WP_023087663.1	hypothetical protein	-
EG09_RS24355	5325819..5326070	+	252	WP_003115979.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T52810 WP_003117425.1 NZ_CP010555:5320698-5320976 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T52810 NZ_CP010555:5320698-5320976 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT52810 WP_003099268.1 NZ_CP010555:5320988-5321293 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT52810 NZ_CP010555:5320988-5321293 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5