Detailed information of TA system TA052797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1080708..1081303
• Replicon: chromosome
• Accession: NZ_CP010554
• Organism: Rugosibacter aromaticivorans strain Ca6

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: PG1C_RS05475
• Protein ID: WP_202636391.1
• Coordinates: 1081022..1081303 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PG1C_RS05470
• Protein ID: WP_202636390.1
• Coordinates: 1080708..1081010 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PG1C_RS05445	1076122..1076466	-	345	WP_202636385.1	hypothetical protein	-
PG1C_RS05450	1076609..1079074	-	2466	WP_202636386.1	DUF3987 domain-containing protein	-
PG1C_RS05455	1079263..1079469	-	207	WP_202636387.1	AlpA family phage regulatory protein	-
PG1C_RS05460	1079546..1080265	-	720	WP_202636388.1	hypothetical protein	-
PG1C_RS05465	1080278..1080445	-	168	WP_202636389.1	hypothetical protein	-
PG1C_RS05470	1080708..1081010	-	303	WP_202636390.1	HigA family addiction module antidote protein	Antitoxin
PG1C_RS05475	1081022..1081303	-	282	WP_202636391.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PG1C_RS05480	1081386..1082591	-	1206	WP_202636392.1	site-specific integrase	-
PG1C_RS05485	1082913..1085576	-	2664	WP_202636393.1	Rne/Rng family ribonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-66)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10655.07 Da
• Isoelectric Point: 7.8610

>T52797 WP_202636391.1 NZ_CP010554:c1081303-1081022 [Rugosibacter aromaticivorans]
MTIRSFRCADTLALYEGRKPKRFRNIEAVAERKLQMLDDAVELRDLASPPGNRLEALGGNRQGQHSIRINNQWRVCFVWA
DNGAADVEIVDYH

Nucleotide

• Length: 282 bp

>T52797 NZ_CP010554:c1081303-1081022 [Rugosibacter aromaticivorans]
ATGACTATCCGCTCTTTCCGATGTGCCGACACACTGGCGCTTTATGAAGGCCGCAAGCCGAAGCGTTTTCGTAATATCGA
AGCCGTTGCCGAACGCAAGCTGCAAATGCTGGATGATGCTGTTGAATTGCGAGACTTGGCATCGCCACCCGGCAACCGGC
TGGAAGCATTGGGCGGCAACCGGCAGGGGCAACATAGCATCCGCATCAATAACCAATGGCGCGTGTGCTTTGTGTGGGCA
GATAACGGTGCGGCGGATGTCGAGATTGTCGATTACCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11341.04 Da
• Isoelectric Point: 8.6220

>AT52797 WP_202636390.1 NZ_CP010554:c1081010-1080708 [Rugosibacter aromaticivorans]
MATNKMRAIHPGEIIREEYLAPLNMSPHALAIELRVPATRLNDIVRERRAISPDTALRLARYFGTTGQFWLNLQGTFDLK
QAESELGQRINQEVKPLEAA

Nucleotide

• Length: 303 bp

>AT52797 NZ_CP010554:c1081010-1080708 [Rugosibacter aromaticivorans]
ATGGCAACGAACAAGATGAGAGCAATACATCCCGGCGAAATTATCCGCGAGGAATATCTCGCCCCCTTGAACATGAGTCC
GCATGCGCTGGCGATTGAGCTGCGCGTACCCGCAACACGCCTCAATGACATTGTGCGCGAACGCCGTGCCATAAGCCCGG
ATACCGCTTTGCGGCTGGCAAGATACTTCGGCACCACGGGGCAATTCTGGCTGAACCTGCAAGGCACGTTTGACCTGAAA
CAGGCAGAGAGCGAGCTAGGGCAACGGATCAATCAGGAAGTGAAGCCGTTAGAGGCGGCGTAA