Detailed information of TA system TA052796

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 609321..609898
• Replicon: chromosome
• Accession: NZ_CP010552
• Organism: Candidatus Thioglobus autotrophicus strain EF1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A0M4P8N6
• Locus tag: SP60_RS03315
• Protein ID: WP_053951275.1
• Coordinates: 609617..609898 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SP60_RS03310
• Protein ID: WP_053951274.1
• Coordinates: 609321..609602 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SP60_RS03285	604814..607408	+	2595	WP_053951270.1	bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase	-
SP60_RS03290	607507..607662	-	156	WP_053951271.1	50S ribosomal protein L33	-
SP60_RS03295	607672..607908	-	237	WP_053951272.1	50S ribosomal protein L28	-
SP60_RS03305	608343..609296	-	954	WP_053951273.1	tyrosine-type recombinase/integrase	-
SP60_RS03310	609321..609602	-	282	WP_053951274.1	HigA family addiction module antidote protein	Antitoxin
SP60_RS03315	609617..609898	-	282	WP_053951275.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SP60_RS03320	610211..610687	+	477	WP_082319678.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
SP60_RS03325	610767..611171	+	405	WP_053951277.1	type II secretion system GspH family protein	-
SP60_RS03330	611176..612414	-	1239	WP_053951278.1	type II secretion system F family protein	-
SP60_RS03335	612431..614128	-	1698	WP_053951279.1	Flp pilus assembly complex ATPase component TadA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-66)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10627.22 Da
• Isoelectric Point: 9.4489

>T52796 WP_053951275.1 NZ_CP010552:c609898-609617 [Candidatus Thioglobus autotrophicus]
MIKSYADKRTEKFATDGKHKSIPSHIIKRALMRLVQIDSATCLNDLCLPSSNHLEGLFGDRKGQSGIRINQQYRVFFIFI
GGNAYDVEIVNYY

Nucleotide

• Length: 282 bp

>T52796 NZ_CP010552:c609898-609617 [Candidatus Thioglobus autotrophicus]
ATGATAAAAAGTTACGCTGACAAAAGAACTGAAAAATTTGCAACTGATGGCAAGCATAAATCGATTCCTAGCCATATTAT
TAAAAGAGCATTAATGAGATTGGTGCAAATAGACAGTGCTACTTGTTTAAATGATTTGTGTTTGCCATCATCAAATCACT
TAGAAGGATTATTTGGCGATAGAAAGGGGCAGAGCGGTATAAGGATTAACCAACAGTACAGGGTATTTTTTATTTTTATA
GGCGGCAATGCTTATGATGTTGAAATTGTTAATTACTATTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10986.35 Da
• Isoelectric Point: 7.1606

>AT52796 WP_053951274.1 NZ_CP010552:c609602-609321 [Candidatus Thioglobus autotrophicus]
MIKNNFHPGEFLQELLEDYNITAYRLSKDIFVQQTRISDILKGRRSISSDTAIRLGKYFNNSPQYWLNLQDSYDLTNCNV
ENYHSINPIQRVV

Nucleotide

• Length: 282 bp

>AT52796 NZ_CP010552:c609602-609321 [Candidatus Thioglobus autotrophicus]
ATGATTAAAAATAACTTCCATCCAGGTGAATTCTTACAAGAGTTGCTAGAAGATTACAACATTACTGCTTATCGCCTATC
TAAAGATATTTTTGTGCAACAGACGCGCATTAGTGACATCTTAAAAGGAAGGCGATCAATTAGTAGTGATACGGCAATCA
GACTTGGTAAATACTTTAACAACTCCCCGCAATATTGGCTTAATTTACAAGACAGCTATGATTTAACTAATTGTAATGTT
GAAAATTACCACAGTATTAATCCTATTCAAAGGGTTGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0M4P8N6