Detailed information of TA system TA052783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2515443..2515960
• Replicon: chromosome
• Accession: NZ_CP010526
• Organism: Staphylococcus aureus subsp. aureus ST772-MRSA-V strain DAR4145

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: RU53_RS12785
• Protein ID: WP_000113262.1
• Coordinates: 2515443..2515709 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: RU53_RS12790
• Protein ID: WP_000584499.1
• Coordinates: 2515709..2515960 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RU53_RS12760	2510867..2511403	-	537	WP_000169317.1	GNAT family N-acetyltransferase	-
RU53_RS12765	2511637..2512461	-	825	WP_000594630.1	formate/nitrite transporter family protein	-
RU53_RS12770	2512677..2512862	-	186	WP_000230293.1	hypothetical protein	-
RU53_RS12775	2512946..2513413	-	468	WP_000153344.1	SRPBCC domain-containing protein	-
RU53_RS12780	2513599..2515149	-	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
RU53_RS12785	2515443..2515709	-	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
RU53_RS12790	2515709..2515960	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RU53_RS15820	2516125..2516226	-	102	WP_031867537.1	hypothetical protein	-
RU53_RS12800	2516233..2516832	-	600	WP_000162799.1	DsbA family protein	-
RU53_RS12805	2516851..2517213	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
RU53_RS12810	2517468..2518718	+	1251	WP_001012219.1	FemA/FemB family glycyltransferase FmhA	-
RU53_RS12815	2518815..2519546	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
RU53_RS12820	2519543..2520262	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T52783 WP_000113262.1 NZ_CP010526:c2515709-2515443 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T52783 NZ_CP010526:c2515709-2515443 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT52783 WP_000584499.1 NZ_CP010526:c2515960-2515709 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT52783 NZ_CP010526:c2515960-2515709 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60