Detailed information of TA system TA052775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1628006..1628429
• Replicon: chromosome
• Accession: NZ_CP010526
• Organism: Staphylococcus aureus subsp. aureus ST772-MRSA-V strain DAR4145

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: RU53_RS08015
• Protein ID: WP_001837494.1
• Coordinates: 1628178..1628429 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0E0VQM8
• Locus tag: RU53_RS08010
• Protein ID: WP_000028422.1
• Coordinates: 1628006..1628188 (-)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RU53_RS07960	1623150..1624811	-	1662	WP_000625088.1	terminase large subunit	-
RU53_RS07965	1624808..1625152	-	345	WP_000402904.1	hypothetical protein	-
RU53_RS07970	1625282..1625581	-	300	WP_000988336.1	HNH endonuclease	-
RU53_RS07975	1625813..1626229	-	417	WP_000590122.1	hypothetical protein	-
RU53_RS07980	1626257..1626457	-	201	WP_000265042.1	DUF1514 family protein	-
RU53_RS07985	1626457..1626606	-	150	WP_000595265.1	transcriptional activator RinB	-
RU53_RS07990	1626603..1626989	-	387	WP_000592207.1	hypothetical protein	-
RU53_RS07995	1626986..1627192	-	207	WP_000195803.1	DUF1381 domain-containing protein	-
RU53_RS08000	1627189..1627434	-	246	WP_001282074.1	hypothetical protein	-
RU53_RS08005	1627471..1628013	-	543	WP_000185660.1	dUTP pyrophosphatase	-
RU53_RS08010	1628006..1628188	-	183	WP_000028422.1	hypothetical protein	Antitoxin
RU53_RS08015	1628178..1628429	-	252	WP_001837494.1	DUF1024 family protein	Toxin
RU53_RS08020	1628422..1628706	-	285	WP_001105620.1	hypothetical protein	-
RU53_RS08025	1628703..1629152	-	450	WP_000982708.1	hypothetical protein	-
RU53_RS08030	1629217..1629459	-	243	WP_000131386.1	phi PVL orf 51-like protein	-
RU53_RS08035	1629463..1629819	-	357	WP_000029376.1	hypothetical protein	-
RU53_RS08040	1629820..1630005	-	186	WP_001187245.1	DUF3113 family protein	-
RU53_RS08045	1630010..1630414	-	405	WP_000049795.1	DUF1064 domain-containing protein	-
RU53_RS08050	1630424..1630645	-	222	WP_001123695.1	DUF3269 family protein	-
RU53_RS15310	1630659..1630817	-	159	WP_000256597.1	hypothetical protein	-
RU53_RS08060	1630811..1631590	-	780	WP_000803063.1	ATP-binding protein	-
RU53_RS08065	1631600..1632370	-	771	WP_000190254.1	conserved phage C-terminal domain-containing protein	-
RU53_RS08070	1632436..1632717	+	282	WP_000414755.1	hypothetical protein	-
RU53_RS16290	1632710..1632859	-	150	WP_001081076.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	lukF-PV / lukS-PV / sea / gnd	1596900..1666620	69720

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9350.20 Da
• Isoelectric Point: 3.9022

>T52775 WP_001837494.1 NZ_CP010526:c1628429-1628178 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
MDNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGMTNAIQHSVKEGVELDEAVGIMAGQVVYKYEEEQE
NEH

Nucleotide

• Length: 252 bp

>T52775 NZ_CP010526:c1628429-1628178 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
ATGGATAACCGTGAACAAATAGAACAATCAGTGATCAGTGCTAGTGCGTATAACGGCAATGACACAGAGGGATTGCTAAA
AGAGATTGAAGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGCATTAG

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6741.63 Da
• Isoelectric Point: 4.2658

>AT52775 WP_000028422.1 NZ_CP010526:c1628188-1628006 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
MSISVGDKVFNPETNSTLEIVQLVGDIRDTHYKLSDGSIISLIDFVVKPIHLIKEEQEND

Nucleotide

• Length: 183 bp

>AT52775 NZ_CP010526:c1628188-1628006 [Staphylococcus aureus subsp. aureus ST772-MRSA-V]
ATGAGCATTAGTGTAGGAGACAAGGTTTTTAATCCAGAAACAAATTCAACTTTAGAAATTGTACAACTTGTTGGCGATAT
TAGAGACACGCATTACAAGTTATCTGACGGATCTATTATTAGTCTTATAGACTTTGTTGTTAAACCAATTCATTTAATCA
AGGAGGAGCAGGAAAATGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VQM8