Detailed information of TA system TA052759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4085664..4086261
• Replicon: chromosome
• Accession: NZ_CP010523
• Organism: Klebsiella variicola strain DSM 15968

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: SP68_RS19155
• Protein ID: WP_012542526.1
• Coordinates: 4085944..4086261 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SP68_RS19150
• Protein ID: WP_012542525.1
• Coordinates: 4085664..4085951 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SP68_RS19120	4081302..4081550	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
SP68_RS19125	4081567..4081908	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
SP68_RS19130	4081939..4083054	-	1116	WP_162499993.1	MBL fold metallo-hydrolase	-
SP68_RS19135	4083234..4083815	+	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
SP68_RS19140	4083815..4084183	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
SP68_RS19145	4084303..4084956	+	654	WP_032729541.1	oxygen-insensitive NAD(P)H nitroreductase	-
SP68_RS19150	4085664..4085951	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
SP68_RS19155	4085944..4086261	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SP68_RS19160	4086446..4087489	-	1044	WP_016160437.1	DUF2157 domain-containing protein	-
SP68_RS19170	4088019..4088885	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
SP68_RS19175	4088994..4090421	+	1428	WP_040973773.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T52759 WP_012542526.1 NZ_CP010523:c4086261-4085944 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T52759 NZ_CP010523:c4086261-4085944 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGTTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCTCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT52759 WP_012542525.1 NZ_CP010523:c4085951-4085664 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT52759 NZ_CP010523:c4085951-4085664 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2