Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2513521..2514148 Replicon chromosome
Accession NZ_CP010516
Organism Cupriavidus gilardii CR3

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag CR3_RS25015 Protein ID WP_082371643.1
Coordinates 2513521..2513802 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag CR3_RS11045 Protein ID WP_053823170.1
Coordinates 2513834..2514148 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR3_RS11020 2508841..2509518 + 678 WP_053822304.1 ATP-binding cassette domain-containing protein -
CR3_RS11025 2509615..2510433 - 819 WP_053822305.1 TOBE domain-containing protein -
CR3_RS11030 2510615..2511751 + 1137 WP_053822306.1 HPP family protein -
CR3_RS11035 2512065..2512759 + 695 Protein_2233 transglycosylase domain-containing protein -
CR3_RS11040 2512910..2513377 + 468 WP_053822307.1 MgtC/SapB family protein -
CR3_RS25015 2513521..2513802 + 282 WP_082371643.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CR3_RS11045 2513834..2514148 + 315 WP_053823170.1 HigA family addiction module antidote protein Antitoxin
CR3_RS11050 2514204..2514425 - 222 WP_053822308.1 formate dehydrogenase subunit delta -
CR3_RS11055 2514428..2515234 - 807 WP_053823171.1 formate dehydrogenase accessory sulfurtransferase FdhD -
CR3_RS11060 2515338..2518220 - 2883 WP_053822309.1 formate dehydrogenase subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - htpB / sodB 2378755..2575115 196360


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(22-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-68)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10702.35 Da        Isoelectric Point: 8.7701

>T52741 WP_082371643.1 NZ_CP010516:2513521-2513802 [Cupriavidus gilardii CR3]
MIQSFRCMDTRALFEGKRVARFTNIHAVAERKLQMLESATTLASLMAPPGNRLEILSGNRRGQYSIRVNAQWRICFLWTS
EGPQCVEIVDYHD

Download         Length: 282 bp

>T52741 NZ_CP010516:2513521-2513802 [Cupriavidus gilardii CR3]
ATGATCCAATCATTCCGATGCATGGACACGCGAGCCCTCTTTGAAGGCAAGCGCGTTGCACGTTTTACCAACATCCACGC
AGTTGCGGAACGCAAACTGCAGATGTTGGAGTCCGCCACCACACTGGCCTCGTTAATGGCTCCGCCAGGCAATCGGCTGG
AAATCCTCTCGGGCAATCGACGGGGCCAGTACAGCATCAGGGTGAACGCCCAATGGCGGATTTGTTTCCTCTGGACCTCC
GAAGGTCCACAGTGCGTAGAAATTGTGGACTACCATGATTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11453.02 Da        Isoelectric Point: 5.1514

>AT52741 WP_053823170.1 NZ_CP010516:2513834-2514148 [Cupriavidus gilardii CR3]
MARFHNGMRPVHPGEILREEYLVPLDLSIAELAKELHIDTAQIDAIVRGDAGVASEIALRLARYFGGDAETWLNLQQAYD
LKTAEQQVGDAIRSEVAPRKAKAA

Download         Length: 315 bp

>AT52741 NZ_CP010516:2513834-2514148 [Cupriavidus gilardii CR3]
ATGGCGCGTTTTCACAACGGCATGCGCCCGGTTCATCCGGGCGAGATCCTGCGCGAGGAGTATCTGGTGCCGCTCGATCT
ATCGATCGCGGAACTGGCGAAGGAATTGCATATCGACACCGCGCAGATCGACGCGATCGTCCGGGGCGATGCCGGCGTGG
CCAGCGAGATCGCTCTGCGTTTGGCGCGCTATTTTGGTGGTGACGCCGAAACCTGGTTGAACCTTCAGCAGGCCTACGAT
CTCAAGACCGCCGAACAGCAGGTCGGCGACGCCATTCGTTCCGAAGTCGCGCCGCGCAAGGCCAAGGCGGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References