Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2886002..2886504 Replicon chromosome
Accession NZ_CP010445
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag SF31_RS14460 Protein ID WP_000767829.1
Coordinates 2886002..2886256 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag SF31_RS14465 Protein ID WP_001259255.1
Coordinates 2886253..2886504 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF31_RS14435 2881017..2881244 - 228 WP_000234896.1 sulfurtransferase TusA -
SF31_RS14440 2881258..2882316 - 1059 WP_000492339.1 transport protein YeeE -
SF31_RS14445 2882495..2883853 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
SF31_RS26055 2883843..2883905 - 63 WP_010723108.1 membrane protein YoeI -
SF31_RS14450 2884120..2885049 - 930 WP_000803351.1 LysR family transcriptional regulator -
SF31_RS14455 2885095..2885919 - 825 WP_000754737.1 SDR family oxidoreductase -
SF31_RS14460 2886002..2886256 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
SF31_RS14465 2886253..2886504 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
SF31_RS24610 2886787..2886837 + 51 WP_001364200.1 his operon leader peptide -
SF31_RS14470 2886983..2887882 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
SF31_RS14475 2887888..2889192 + 1305 WP_000009594.1 histidinol dehydrogenase -
SF31_RS14480 2889189..2890259 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
SF31_RS14485 2890259..2891326 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T52724 WP_000767829.1 NZ_CP010445:c2886256-2886002 [Escherichia coli K-12]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T52724 NZ_CP010445:c2886256-2886002 [Escherichia coli K-12]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT52724 WP_001259255.1 NZ_CP010445:c2886504-2886253 [Escherichia coli K-12]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT52724 NZ_CP010445:c2886504-2886253 [Escherichia coli K-12]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References