Detailed information of TA system TA052701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1066001..1066695
• Replicon: chromosome
• Accession: NZ_CP010445
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: SF31_RS05240
• Protein ID: WP_001263489.1
• Coordinates: 1066297..1066695 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: SF31_RS05235
• Protein ID: WP_000554758.1
• Coordinates: 1066001..1066294 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SF31_RS05215	1061633..1062130	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
SF31_RS05220	1062354..1064066	-	1713	Protein_962	flagellar biosynthesis protein FlhA	-
SF31_RS05225	1064038..1064823	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
SF31_RS05230	1064894..1065949	+	1056	WP_001226164.1	DNA polymerase IV	-
SF31_RS05235	1066001..1066294	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
SF31_RS05240	1066297..1066695	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
SF31_RS05245	1066705..1067157	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
SF31_RS05250	1067475..1067681	+	207	Protein_968	RtcB family protein	-
SF31_RS05255	1067677..1068198	+	522	Protein_969	peptide chain release factor H	-
SF31_RS05260	1068255..1069712	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
SF31_RS05265	1069973..1070431	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	1071027..1071107	+	81	NuclAT_12	-	-
-	1071027..1071107	+	81	NuclAT_12	-	-
-	1071027..1071107	+	81	NuclAT_12	-	-
-	1071027..1071107	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T52701 WP_001263489.1 NZ_CP010445:1066297-1066695 [Escherichia coli K-12]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T52701 NZ_CP010445:1066297-1066695 [Escherichia coli K-12]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT52701 WP_000554758.1 NZ_CP010445:1066001-1066294 [Escherichia coli K-12]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT52701 NZ_CP010445:1066001-1066294 [Escherichia coli K-12]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3