Detailed information of TA system TA052648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4014506..4015305
• Replicon: chromosome
• Accession: NZ_CP010443
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: SH04_RS19815
• Protein ID: WP_000347273.1
• Coordinates: 4014841..4015305 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: SH04_RS19810
• Protein ID: WP_001307405.1
• Coordinates: 4014506..4014841 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH04_RS19795	4010291..4011061	-	771	WP_001058209.1	2-dehydro-3-deoxyglucarate aldolase	-
SH04_RS19800	4011077..4012411	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
SH04_RS19805	4012786..4014357	+	1572	WP_001273753.1	galactarate dehydratase	-
SH04_RS19810	4014506..4014841	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
SH04_RS19815	4014841..4015305	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
SH04_RS19820	4015360..4016169	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
SH04_RS19825	4016418..4017698	+	1281	WP_000681920.1	tagatose-bisphosphate aldolase subunit KbaZ	-
SH04_RS19830	4017721..4018194	+	474	WP_001336162.1	PTS N-acetylgalactosamine transporter subunit IIB	-
SH04_RS19835	4018205..4018576	+	372	Protein_3750	PTS sugar transporter subunit IIC	-
SH04_RS19840	4018572..4019129	+	558	Protein_3751	amidohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3999305..4026289	26984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T52648 WP_000347273.1 NZ_CP010443:4014841-4015305 [Escherichia coli K-12]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T52648 NZ_CP010443:4014841-4015305 [Escherichia coli K-12]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT52648 WP_001307405.1 NZ_CP010443:4014506-4014841 [Escherichia coli K-12]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT52648 NZ_CP010443:4014506-4014841 [Escherichia coli K-12]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: A0A0E0XVC7