Detailed information of TA system TA052625

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2156353..2156724
• Replicon: chromosome
• Accession: NZ_CP010443
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: SH04_RS10675
• Protein ID: WP_001317028.1
• Coordinates: 2156530..2156724 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2156353..2156531 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH04_RS10650	2152308..2153681	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
SH04_RS10655	2153810..2154745	-	936	WP_001157406.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
SH04_RS23895	2154797..2156032	-	1236	WP_000040852.1	site-specific integrase	-
SH04_RS10665	2156034..2156249	-	216	WP_000079604.1	excisionase XisR	-
SH04_RS10670	2156328..2156537	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	2156353..2156531	+	179	NuclAT_0	-	Antitoxin
-	2156353..2156531	+	179	NuclAT_0	-	Antitoxin
-	2156353..2156531	+	179	NuclAT_0	-	Antitoxin
-	2156353..2156531	+	179	NuclAT_0	-	Antitoxin
SH04_RS10675	2156530..2156724	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
SH04_RS10680	2156781..2157590	-	810	WP_000166319.1	recombination protein RecT	-
SH04_RS10685	2157583..2160183	-	2601	WP_000105143.1	exodeoxyribonuclease VIII	-
SH04_RS10690	2160285..2160560	-	276	WP_000632297.1	protein RacC	-
SH04_RS10695	2160635..2160805	-	171	WP_001352098.1	conserved protein, Rac prophage	-
SH04_RS10700	2160805..2161026	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2123618..2176471	52853

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T52625 WP_001317028.1 NZ_CP010443:c2156724-2156530 [Escherichia coli K-12]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T52625 NZ_CP010443:c2156724-2156530 [Escherichia coli K-12]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT52625 NZ_CP010443:2156353-2156531 [Escherichia coli K-12]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin