Detailed information of TA system TA052572

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-ohsC/SymE(toxin)
• Location: 749919..750331
• Replicon: chromosome
• Accession: NZ_CP010442
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: SH06_RS03735
• Protein ID: WP_000132601.1
• Coordinates: 749919..750260 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	ohsC
Locus tag	-
Coordinates	750255..750331 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH06_RS03725	747332..748378	-	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-
SH06_RS03730	748378..749757	-	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
SH06_RS03735	749919..750260	-	342	WP_000132601.1	endoribonuclease SymE	Toxin
-	750255..750331	+	77	NuclAT_14	-	Antitoxin
-	750255..750331	+	77	NuclAT_14	-	Antitoxin
-	750255..750331	+	77	NuclAT_14	-	Antitoxin
-	750255..750331	+	77	NuclAT_14	-	Antitoxin
-	750255..750331	+	77	NuclAT_15	-	Antitoxin
-	750255..750331	+	77	NuclAT_15	-	Antitoxin
-	750255..750331	+	77	NuclAT_15	-	Antitoxin
-	750255..750331	+	77	NuclAT_15	-	Antitoxin
SH06_RS03740	750488..751882	-	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
SH06_RS03745	751879..753468	-	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	tet(B)	-	742834..760207	17373

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T52572 WP_000132601.1 NZ_CP010442:c750260-749919 [Escherichia coli K-12]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T52572 NZ_CP010442:c750260-749919 [Escherichia coli K-12]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT52572 NZ_CP010442:750255-750331 [Escherichia coli K-12]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin