Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2832005..2832507 Replicon chromosome
Accession NZ_CP010441
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag SH03_RS14145 Protein ID WP_000767829.1
Coordinates 2832005..2832259 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag SH03_RS14150 Protein ID WP_001259255.1
Coordinates 2832256..2832507 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH03_RS14120 2827020..2827247 - 228 WP_000234896.1 sulfurtransferase TusA -
SH03_RS14125 2827261..2828319 - 1059 WP_000492339.1 transport protein YeeE -
SH03_RS14130 2828498..2829856 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
SH03_RS25625 2829846..2829908 - 63 WP_010723108.1 membrane protein YoeI -
SH03_RS14135 2830123..2831052 - 930 WP_000803351.1 LysR family transcriptional regulator -
SH03_RS14140 2831098..2831922 - 825 WP_000754737.1 SDR family oxidoreductase -
SH03_RS14145 2832005..2832259 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
SH03_RS14150 2832256..2832507 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
SH03_RS24200 2832790..2832840 + 51 WP_001364200.1 his operon leader peptide -
SH03_RS14155 2832986..2833849 + 864 WP_038432600.1 ATP phosphoribosyltransferase -
SH03_RS14160 2833855..2835159 + 1305 WP_000009594.1 histidinol dehydrogenase -
SH03_RS14165 2835156..2836226 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
SH03_RS14170 2836226..2837293 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T52558 WP_000767829.1 NZ_CP010441:c2832259-2832005 [Escherichia coli K-12]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T52558 NZ_CP010441:c2832259-2832005 [Escherichia coli K-12]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT52558 WP_001259255.1 NZ_CP010441:c2832507-2832256 [Escherichia coli K-12]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT52558 NZ_CP010441:c2832507-2832256 [Escherichia coli K-12]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References