52558

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2832005..2832507 Replicon chromosome
Accession NZ_CP010441
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag SH03_RS14145 Protein ID WP_000767829.1
Coordinates 2832005..2832259 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag SH03_RS14150 Protein ID WP_001259255.1
Coordinates 2832256..2832507 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH03_RS14120 2827020..2827247 - 228 WP_000234896.1 sulfurtransferase TusA -
SH03_RS14125 2827261..2828319 - 1059 WP_000492339.1 transport protein YeeE -
SH03_RS14130 2828498..2829856 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
SH03_RS25625 2829846..2829908 - 63 WP_010723108.1 membrane protein YoeI -
SH03_RS14135 2830123..2831052 - 930 WP_000803351.1 LysR family transcriptional regulator -
SH03_RS14140 2831098..2831922 - 825 WP_000754737.1 SDR family oxidoreductase -
SH03_RS14145 2832005..2832259 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
SH03_RS14150 2832256..2832507 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
SH03_RS24200 2832790..2832840 + 51 WP_001364200.1 his operon leader peptide -
SH03_RS14155 2832986..2833849 + 864 WP_038432600.1 ATP phosphoribosyltransferase -
SH03_RS14160 2833855..2835159 + 1305 WP_000009594.1 histidinol dehydrogenase -
SH03_RS14165 2835156..2836226 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
SH03_RS14170 2836226..2837293 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T52558 WP_000767829.1 NZ_CP010441:c2832259-2832005 [Escherichia coli K-12]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T52558 NZ_CP010441:c2832259-2832005 [Escherichia coli K-12]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT52558 WP_001259255.1 NZ_CP010441:c2832507-2832256 [Escherichia coli K-12]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT52558 NZ_CP010441:c2832507-2832256 [Escherichia coli K-12]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References