Detailed information of TA system TA052522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3918463..3919156
• Replicon: chromosome
• Accession: NZ_CP010440
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: SH08_RS19365
• Protein ID: WP_000415584.1
• Coordinates: 3918860..3919156 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: SH08_RS19360
• Protein ID: WP_000650107.1
• Coordinates: 3918463..3918858 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH08_RS19350	3914328..3916585	-	2258	Protein_3663	DNA topoisomerase IV subunit A	-
SH08_RS19355	3916723..3918330	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
SH08_RS19360	3918463..3918858	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
SH08_RS19365	3918860..3919156	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
SH08_RS19370	3919361..3919843	-	483	WP_000183505.1	transcriptional regulator	-
SH08_RS19375	3919896..3920288	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
SH08_RS19380	3920440..3921099	+	660	WP_001221495.1	two-component system response regulator QseB	-
SH08_RS19385	3921096..3922445	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
SH08_RS19390	3922491..3922823	-	333	WP_000917684.1	DUF2645 family protein	-
SH08_RS19395	3923142..3923723	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
SH08_RS19400	3923754..3924068	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T52522 WP_000415584.1 NZ_CP010440:c3919156-3918860 [Escherichia coli K-12]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T52522 NZ_CP010440:c3919156-3918860 [Escherichia coli K-12]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT52522 WP_000650107.1 NZ_CP010440:c3918858-3918463 [Escherichia coli K-12]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT52522 NZ_CP010440:c3918858-3918463 [Escherichia coli K-12]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65