Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2845542..2846044 Replicon chromosome
Accession NZ_CP010440
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag SH08_RS14240 Protein ID WP_000767829.1
Coordinates 2845542..2845796 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag SH08_RS14245 Protein ID WP_001259255.1
Coordinates 2845793..2846044 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH08_RS14215 2840557..2840784 - 228 WP_000234896.1 sulfurtransferase TusA -
SH08_RS14220 2840798..2841856 - 1059 WP_000492339.1 transport protein YeeE -
SH08_RS14225 2842035..2843393 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
SH08_RS25795 2843383..2843445 - 63 WP_010723108.1 membrane protein YoeI -
SH08_RS14230 2843660..2844589 - 930 WP_000803351.1 LysR family transcriptional regulator -
SH08_RS14235 2844635..2845459 - 825 WP_000754737.1 SDR family oxidoreductase -
SH08_RS14240 2845542..2845796 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
SH08_RS14245 2845793..2846044 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
SH08_RS24335 2846327..2846377 + 51 WP_001364200.1 his operon leader peptide -
SH08_RS14250 2846523..2847386 + 864 WP_038432600.1 ATP phosphoribosyltransferase -
SH08_RS14255 2847392..2848696 + 1305 WP_000009594.1 histidinol dehydrogenase -
SH08_RS14260 2848693..2849763 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
SH08_RS14265 2849763..2850830 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T52517 WP_000767829.1 NZ_CP010440:c2845796-2845542 [Escherichia coli K-12]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T52517 NZ_CP010440:c2845796-2845542 [Escherichia coli K-12]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT52517 WP_001259255.1 NZ_CP010440:c2846044-2845793 [Escherichia coli K-12]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT52517 NZ_CP010440:c2846044-2845793 [Escherichia coli K-12]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References