Detailed information of TA system TA052484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3901841..3902534
• Replicon: chromosome
• Accession: NZ_CP010439
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: SH02_RS19325
• Protein ID: WP_000415584.1
• Coordinates: 3902238..3902534 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: SH02_RS19320
• Protein ID: WP_000650107.1
• Coordinates: 3901841..3902236 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH02_RS19310	3897705..3899963	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
SH02_RS19315	3900101..3901708	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
SH02_RS19320	3901841..3902236	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
SH02_RS19325	3902238..3902534	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
SH02_RS19330	3902739..3903221	-	483	WP_000183505.1	transcriptional regulator	-
SH02_RS19335	3903274..3903666	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
SH02_RS19340	3903818..3904477	+	660	WP_001221495.1	two-component system response regulator QseB	-
SH02_RS19345	3904474..3905823	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
SH02_RS19350	3905869..3906201	-	333	WP_000917684.1	DUF2645 family protein	-
SH02_RS19355	3906520..3907101	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
SH02_RS19360	3907132..3907446	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T52484 WP_000415584.1 NZ_CP010439:c3902534-3902238 [Escherichia coli K-12]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T52484 NZ_CP010439:c3902534-3902238 [Escherichia coli K-12]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT52484 WP_000650107.1 NZ_CP010439:c3902236-3901841 [Escherichia coli K-12]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT52484 NZ_CP010439:c3902236-3901841 [Escherichia coli K-12]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65