Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2828914..2829416 Replicon chromosome
Accession NZ_CP010439
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag SH02_RS14200 Protein ID WP_000767829.1
Coordinates 2828914..2829168 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag SH02_RS14205 Protein ID WP_001259255.1
Coordinates 2829165..2829416 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH02_RS14175 2823929..2824156 - 228 WP_000234896.1 sulfurtransferase TusA -
SH02_RS14180 2824170..2825228 - 1059 WP_000492339.1 transport protein YeeE -
SH02_RS14185 2825407..2826765 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
SH02_RS25725 2826755..2826817 - 63 WP_010723108.1 membrane protein YoeI -
SH02_RS14190 2827032..2827961 - 930 WP_000803351.1 LysR family transcriptional regulator -
SH02_RS14195 2828007..2828831 - 825 WP_000754737.1 SDR family oxidoreductase -
SH02_RS14200 2828914..2829168 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
SH02_RS14205 2829165..2829416 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
SH02_RS24295 2829699..2829749 + 51 WP_001364200.1 his operon leader peptide -
SH02_RS14210 2829895..2830758 + 864 WP_038432600.1 ATP phosphoribosyltransferase -
SH02_RS14215 2830764..2832068 + 1305 WP_000009594.1 histidinol dehydrogenase -
SH02_RS14220 2832065..2833135 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
SH02_RS14225 2833135..2834202 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T52479 WP_000767829.1 NZ_CP010439:c2829168-2828914 [Escherichia coli K-12]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T52479 NZ_CP010439:c2829168-2828914 [Escherichia coli K-12]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT52479 WP_001259255.1 NZ_CP010439:c2829416-2829165 [Escherichia coli K-12]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT52479 NZ_CP010439:c2829416-2829165 [Escherichia coli K-12]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References