Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 993303..993844 Replicon chromosome
Accession NZ_CP010439
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag SH02_RS04850 Protein ID WP_000615983.1
Coordinates 993303..993581 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag SH02_RS04855 Protein ID WP_000729703.1
Coordinates 993584..993844 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH02_RS04835 990885..991463 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
SH02_RS04840 991669..992436 + 768 WP_038432477.1 class II glutamine amidotransferase -
SH02_RS04845 992407..993147 - 741 WP_001225679.1 murein L,D-transpeptidase -
SH02_RS04850 993303..993581 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
SH02_RS04855 993584..993844 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
SH02_RS04860 994054..994803 + 750 WP_000056849.1 C40 family peptidase -
SH02_RS04865 994979..995476 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
SH02_RS04870 995700..997412 - 1713 Protein_896 flagellar biosynthesis protein FlhA -
SH02_RS04875 997384..998169 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T52459 WP_000615983.1 NZ_CP010439:c993581-993303 [Escherichia coli K-12]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T52459 NZ_CP010439:c993581-993303 [Escherichia coli K-12]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT52459 WP_000729703.1 NZ_CP010439:c993844-993584 [Escherichia coli K-12]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT52459 NZ_CP010439:c993844-993584 [Escherichia coli K-12]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References