Detailed information of TA system TA052451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3917027..3917720
• Replicon: chromosome
• Accession: NZ_CP010438
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: SH05_RS19370
• Protein ID: WP_000415584.1
• Coordinates: 3917424..3917720 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: SH05_RS19365
• Protein ID: WP_000650107.1
• Coordinates: 3917027..3917422 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH05_RS19355	3912891..3915149	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
SH05_RS19360	3915287..3916894	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
SH05_RS19365	3917027..3917422	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
SH05_RS19370	3917424..3917720	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
SH05_RS19375	3917925..3918407	-	483	WP_000183505.1	transcriptional regulator	-
SH05_RS19380	3918460..3918852	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
SH05_RS19385	3919004..3919663	+	660	WP_001221495.1	two-component system response regulator QseB	-
SH05_RS19390	3919660..3921009	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
SH05_RS19395	3921055..3921387	-	333	WP_000917684.1	DUF2645 family protein	-
SH05_RS19400	3921706..3922287	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
SH05_RS19405	3922318..3922632	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T52451 WP_000415584.1 NZ_CP010438:c3917720-3917424 [Escherichia coli K-12]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T52451 NZ_CP010438:c3917720-3917424 [Escherichia coli K-12]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT52451 WP_000650107.1 NZ_CP010438:c3917422-3917027 [Escherichia coli K-12]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT52451 NZ_CP010438:c3917422-3917027 [Escherichia coli K-12]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65