Detailed information of TA system TA052377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1840170..1840777
• Replicon: chromosome
• Accession: NZ_CP010415
• Organism: Azotobacter chroococcum NCIMB 8003

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: Achr_RS08385
• Protein ID: WP_039807663.1
• Coordinates: 1840170..1840484 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Achr_RS08390
• Protein ID: WP_012701743.1
• Coordinates: 1840490..1840777 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Achr_RS08365	1836721..1836957	+	237	WP_039803513.1	hypothetical protein	-
Achr_RS08370	1837020..1837511	+	492	WP_039803515.1	glycoside hydrolase family 104 protein	-
Achr_RS08375	1837508..1837951	+	444	WP_039803516.1	holin family protein	-
Achr_RS25745	1838018..1838161	-	144	WP_158319648.1	hypothetical protein	-
Achr_RS23995	1838158..1838262	-	105	WP_082045391.1	toxin-antitoxin system HicB family antitoxin	-
Achr_RS08380	1838355..1839440	+	1086	WP_039807303.1	IS5 family transposase	-
Achr_RS08385	1840170..1840484	+	315	WP_039807663.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Achr_RS08390	1840490..1840777	+	288	WP_012701743.1	helix-turn-helix domain-containing protein	Antitoxin
Achr_RS08395	1840789..1840986	+	198	WP_039803519.1	hypothetical protein	-
Achr_RS08400	1841074..1841469	-	396	WP_039807666.1	hypothetical protein	-
Achr_RS08405	1841733..1841987	+	255	WP_039803521.1	type II toxin-antitoxin system HicA family toxin	-
Achr_RS08410	1841984..1842316	+	333	WP_039803522.1	type II toxin-antitoxin system HicB family antitoxin	-
Achr_RS25750	1842530..1842667	+	138	WP_158319649.1	hypothetical protein	-
Achr_RS08420	1843207..1844478	-	1272	WP_039803524.1	sodium:proton antiporter	-
Achr_RS08425	1844514..1844924	-	411	WP_039803525.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1829714..1849494	19780
	-	flank	IS/Tn	-	-	1838334..1839440	1106

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-87)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11927.77 Da
• Isoelectric Point: 8.8937

>T52377 WP_039807663.1 NZ_CP010415:1840170-1840484 [Azotobacter chroococcum NCIMB 8003]
MIFIETPIFTRCLRELLDDDSYAAFQKMLAERPDVGDVIEGTGGIRKVRVASKGHGKRGGSRVIYYHFVSESQIALLLIY
PKNEKDDLTADERKALKRIIETWR

Nucleotide

• Length: 315 bp

>T52377 NZ_CP010415:1840170-1840484 [Azotobacter chroococcum NCIMB 8003]
ATGATATTCATCGAAACCCCCATATTCACCAGATGCCTGCGCGAGTTGCTGGATGACGACAGCTATGCAGCCTTCCAGAA
GATGCTGGCCGAGCGCCCCGATGTCGGCGATGTCATCGAGGGCACTGGAGGCATTCGGAAGGTGCGTGTCGCCTCGAAGG
GGCATGGAAAACGGGGTGGCTCCCGGGTTATCTACTATCACTTCGTCTCGGAATCGCAGATCGCCCTGCTGCTGATCTAC
CCGAAGAACGAGAAGGATGACCTGACTGCCGACGAGCGCAAAGCGCTCAAACGCATCATCGAGACGTGGAGGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10703.28 Da
• Isoelectric Point: 8.8270

>AT52377 WP_012701743.1 NZ_CP010415:1840490-1840777 [Azotobacter chroococcum NCIMB 8003]
MSKFFDDLMESVVQMDEIVRGERQPSREFTVDALQVKQIRKATGLSQAKFAAMIDVQVGTLRNWEQGRREPTGPAKALLR
AIHNDPEHVIRALAG

Nucleotide

• Length: 288 bp

>AT52377 NZ_CP010415:1840490-1840777 [Azotobacter chroococcum NCIMB 8003]
ATGAGTAAGTTTTTCGACGATCTCATGGAAAGCGTCGTGCAAATGGACGAAATCGTTCGCGGCGAACGCCAACCGTCCCG
CGAGTTCACCGTGGATGCCTTGCAGGTGAAGCAGATCCGCAAGGCCACCGGTCTTTCGCAGGCCAAGTTTGCCGCGATGA
TCGATGTGCAGGTGGGCACGCTGCGCAACTGGGAACAAGGCCGCCGCGAACCGACCGGCCCGGCCAAGGCTCTGCTGCGC
GCCATCCACAATGACCCGGAGCACGTCATTCGAGCGCTGGCGGGCTAA