Detailed information of TA system TA052375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 363210..363865
• Replicon: chromosome
• Accession: NZ_CP010415
• Organism: Azotobacter chroococcum NCIMB 8003

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0C4WP14
• Locus tag: Achr_RS01705
• Protein ID: WP_039807391.1
• Coordinates: 363210..363554 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Achr_RS01710
• Protein ID: WP_039801235.1
• Coordinates: 363551..363865 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Achr_RS01685	359038..359790	+	753	WP_039801226.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
Achr_RS01690	359824..361185	+	1362	WP_039801229.1	glutamine synthetase family protein	-
Achr_RS01695	361248..362621	+	1374	WP_039801231.1	aspartate aminotransferase family protein	-
Achr_RS01700	362758..363000	+	243	WP_039801233.1	transposase	-
Achr_RS01705	363210..363554	+	345	WP_039807391.1	hypothetical protein	Toxin
Achr_RS01710	363551..363865	+	315	WP_039801235.1	helix-turn-helix domain-containing protein	Antitoxin
Achr_RS24900	364432..364947	+	516	WP_144411493.1	hypothetical protein	-
Achr_RS24905	365040..365528	+	489	WP_144411494.1	hypothetical protein	-
Achr_RS01715	365635..366123	+	489	WP_039801238.1	hypothetical protein	-
Achr_RS01720	366607..367761	+	1155	WP_193388179.1	polyamine ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (42-88)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13082.01 Da
• Isoelectric Point: 9.6389

>T52375 WP_039807391.1 NZ_CP010415:363210-363554 [Azotobacter chroococcum NCIMB 8003]
MKAIFIETTSFTATVGDYLTDDEYRQLQGELLKTPEAGDVMPRTGGFRKLRWADGRRGKGKRGGLRVIYYWLLGDGQFWM
FAIYDKDELENLTAEQEKLLKQAITAELKKRGAK

Nucleotide

• Length: 345 bp

>T52375 NZ_CP010415:363210-363554 [Azotobacter chroococcum NCIMB 8003]
ATGAAAGCCATCTTCATCGAAACCACGAGCTTCACCGCCACGGTCGGCGACTACCTGACCGACGACGAGTATCGGCAGCT
CCAGGGGGAACTGCTGAAGACCCCGGAAGCCGGCGACGTCATGCCGCGCACGGGCGGCTTCCGCAAGCTGCGCTGGGCCG
ATGGACGCAGAGGGAAGGGAAAGCGTGGTGGCTTGCGCGTCATCTACTACTGGCTGCTCGGCGATGGCCAGTTCTGGATG
TTCGCCATCTACGACAAGGACGAGCTGGAGAACCTGACCGCCGAGCAGGAGAAGCTGCTCAAGCAGGCCATCACCGCAGA
GCTGAAAAAGCGAGGTGCCAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11951.98 Da
• Isoelectric Point: 10.6449

>AT52375 WP_039801235.1 NZ_CP010415:363551-363865 [Azotobacter chroococcum NCIMB 8003]
VKKRDLFAEMMQGIGEMAAHREGKITLRQYEVEERPAPEVSASEIIALRKKMHMSQQVFARRFRISTGTLRKWEQAKAKP
NAQAAVLIRLVEQYPDMAERLAAI

Nucleotide

• Length: 315 bp

>AT52375 NZ_CP010415:363551-363865 [Azotobacter chroococcum NCIMB 8003]
GTGAAAAAGCGTGATTTGTTTGCCGAGATGATGCAGGGCATCGGCGAAATGGCGGCGCACCGCGAGGGCAAGATCACCCT
GCGCCAATACGAGGTCGAGGAAAGGCCGGCGCCGGAGGTGTCGGCGAGCGAGATCATCGCCCTGCGCAAGAAAATGCACA
TGTCCCAGCAGGTGTTTGCCCGCCGCTTCCGCATCAGCACGGGCACCCTGCGCAAATGGGAGCAGGCCAAGGCCAAGCCG
AACGCCCAGGCCGCCGTTCTCATCAGGCTGGTCGAGCAGTACCCCGACATGGCCGAAAGACTGGCCGCGATCTGA

Structures

Toxin

• AlphaFold DB: A0A0C4WP14