Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 76845..77536 Replicon chromosome
Accession NZ_CP010415
Organism Azotobacter chroococcum NCIMB 8003

Toxin (Protein)


Gene name higB Uniprot ID A0A0C4WKM4
Locus tag Achr_RS00320 Protein ID WP_039800697.1
Coordinates 77249..77536 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0C4WIL7
Locus tag Achr_RS00315 Protein ID WP_039800694.1
Coordinates 76845..77246 (-) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Achr_RS24850 72125..72475 + 351 WP_144411486.1 hypothetical protein -
Achr_RS24855 72468..72773 + 306 WP_144411487.1 hypothetical protein -
Achr_RS23645 72925..74076 + 1152 WP_082045335.1 hypothetical protein -
Achr_RS23650 74174..74677 + 504 Protein_67 metal-dependent hydrolase -
Achr_RS00305 74745..75830 + 1086 WP_039807303.1 IS5 family transposase -
Achr_RS00310 76160..76588 + 429 WP_039800691.1 SgcJ/EcaC family oxidoreductase -
Achr_RS00315 76845..77246 - 402 WP_039800694.1 helix-turn-helix domain-containing protein Antitoxin
Achr_RS00320 77249..77536 - 288 WP_039800697.1 type II toxin-antitoxin system HigB family toxin Toxin
Achr_RS00325 77819..78661 + 843 WP_039800700.1 alpha/beta hydrolase -
Achr_RS00330 78888..79838 - 951 WP_039800704.1 homoserine kinase -
Achr_RS00335 79855..80142 - 288 WP_039800707.1 DUF2782 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 74724..75830 1106


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-90)

Antitoxin

Cro/C1-type helix-turn-helix domain

(78-132)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11077.78 Da        Isoelectric Point: 9.9973

>T52374 WP_039800697.1 NZ_CP010415:c77536-77249 [Azotobacter chroococcum NCIMB 8003]
MHVITQKRIWEAKAKWPHAATALEAWYRLIKASAPQDFAEMKHLFPATDKVGPQHVFDIGGNKLRLIAVVQYRAQKVYIQ
HVLDHGEYDQGKWRE

Download         Length: 288 bp

>T52374 NZ_CP010415:c77536-77249 [Azotobacter chroococcum NCIMB 8003]
ATGCACGTCATTACTCAGAAACGCATCTGGGAAGCCAAGGCCAAGTGGCCGCATGCGGCCACTGCCTTGGAGGCCTGGTA
TCGCCTAATCAAGGCCAGTGCTCCACAGGACTTCGCGGAGATGAAGCATCTGTTTCCGGCAACAGACAAGGTGGGGCCGC
AGCATGTGTTCGACATCGGCGGCAACAAGCTTCGCCTGATCGCCGTTGTGCAGTACCGGGCGCAGAAGGTCTATATCCAG
CATGTGCTGGATCATGGCGAATACGACCAAGGTAAGTGGAGGGAATAA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14909.97 Da        Isoelectric Point: 4.4693

>AT52374 WP_039800694.1 NZ_CP010415:c77246-76845 [Azotobacter chroococcum NCIMB 8003]
MSALIKQASEHWRYVAPLLTRPANEDDYDALVEALDELLVIVGDDEDHPLATLASHMGDLIEAYDEAQRPMPKVSGVEML
RYLMQEHGLTQADLPEIGVQSVVSELLSGKRRLNVRHIRALSERFGVPADVFF

Download         Length: 402 bp

>AT52374 NZ_CP010415:c77246-76845 [Azotobacter chroococcum NCIMB 8003]
ATGTCCGCGCTGATCAAACAAGCCAGCGAACACTGGCGCTATGTGGCCCCCTTGCTCACCAGGCCGGCCAACGAAGACGA
CTACGACGCCTTGGTCGAAGCCCTGGACGAATTGCTGGTTATCGTCGGCGACGACGAAGATCATCCCCTTGCCACCCTGG
CCTCGCACATGGGCGACCTGATCGAAGCCTACGATGAAGCCCAGCGTCCTATGCCGAAGGTGAGCGGCGTGGAGATGCTG
CGCTACCTGATGCAGGAGCATGGTCTGACTCAGGCCGATCTGCCGGAGATCGGCGTGCAGTCCGTCGTTTCCGAGCTGCT
CAGCGGCAAGCGCCGGCTCAATGTGCGCCATATCCGCGCGTTATCCGAGCGCTTTGGTGTACCGGCGGATGTTTTCTTTT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C4WKM4

Antitoxin

Source ID Structure
AlphaFold DB A0A0C4WIL7

References