Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
Location | 76845..77536 | Replicon | chromosome |
Accession | NZ_CP010415 | ||
Organism | Azotobacter chroococcum NCIMB 8003 |
Toxin (Protein)
Gene name | higB | Uniprot ID | A0A0C4WKM4 |
Locus tag | Achr_RS00320 | Protein ID | WP_039800697.1 |
Coordinates | 77249..77536 (-) | Length | 96 a.a. |
Antitoxin (Protein)
Gene name | higA | Uniprot ID | A0A0C4WIL7 |
Locus tag | Achr_RS00315 | Protein ID | WP_039800694.1 |
Coordinates | 76845..77246 (-) | Length | 134 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
Achr_RS24850 | 72125..72475 | + | 351 | WP_144411486.1 | hypothetical protein | - |
Achr_RS24855 | 72468..72773 | + | 306 | WP_144411487.1 | hypothetical protein | - |
Achr_RS23645 | 72925..74076 | + | 1152 | WP_082045335.1 | hypothetical protein | - |
Achr_RS23650 | 74174..74677 | + | 504 | Protein_67 | metal-dependent hydrolase | - |
Achr_RS00305 | 74745..75830 | + | 1086 | WP_039807303.1 | IS5 family transposase | - |
Achr_RS00310 | 76160..76588 | + | 429 | WP_039800691.1 | SgcJ/EcaC family oxidoreductase | - |
Achr_RS00315 | 76845..77246 | - | 402 | WP_039800694.1 | helix-turn-helix domain-containing protein | Antitoxin |
Achr_RS00320 | 77249..77536 | - | 288 | WP_039800697.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
Achr_RS00325 | 77819..78661 | + | 843 | WP_039800700.1 | alpha/beta hydrolase | - |
Achr_RS00330 | 78888..79838 | - | 951 | WP_039800704.1 | homoserine kinase | - |
Achr_RS00335 | 79855..80142 | - | 288 | WP_039800707.1 | DUF2782 domain-containing protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | flank | IS/Tn | - | - | 74724..75830 | 1106 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11077.78 Da Isoelectric Point: 9.9973
>T52374 WP_039800697.1 NZ_CP010415:c77536-77249 [Azotobacter chroococcum NCIMB 8003]
MHVITQKRIWEAKAKWPHAATALEAWYRLIKASAPQDFAEMKHLFPATDKVGPQHVFDIGGNKLRLIAVVQYRAQKVYIQ
HVLDHGEYDQGKWRE
MHVITQKRIWEAKAKWPHAATALEAWYRLIKASAPQDFAEMKHLFPATDKVGPQHVFDIGGNKLRLIAVVQYRAQKVYIQ
HVLDHGEYDQGKWRE
Download Length: 288 bp
>T52374 NZ_CP010415:c77536-77249 [Azotobacter chroococcum NCIMB 8003]
ATGCACGTCATTACTCAGAAACGCATCTGGGAAGCCAAGGCCAAGTGGCCGCATGCGGCCACTGCCTTGGAGGCCTGGTA
TCGCCTAATCAAGGCCAGTGCTCCACAGGACTTCGCGGAGATGAAGCATCTGTTTCCGGCAACAGACAAGGTGGGGCCGC
AGCATGTGTTCGACATCGGCGGCAACAAGCTTCGCCTGATCGCCGTTGTGCAGTACCGGGCGCAGAAGGTCTATATCCAG
CATGTGCTGGATCATGGCGAATACGACCAAGGTAAGTGGAGGGAATAA
ATGCACGTCATTACTCAGAAACGCATCTGGGAAGCCAAGGCCAAGTGGCCGCATGCGGCCACTGCCTTGGAGGCCTGGTA
TCGCCTAATCAAGGCCAGTGCTCCACAGGACTTCGCGGAGATGAAGCATCTGTTTCCGGCAACAGACAAGGTGGGGCCGC
AGCATGTGTTCGACATCGGCGGCAACAAGCTTCGCCTGATCGCCGTTGTGCAGTACCGGGCGCAGAAGGTCTATATCCAG
CATGTGCTGGATCATGGCGAATACGACCAAGGTAAGTGGAGGGAATAA
Antitoxin
Download Length: 134 a.a. Molecular weight: 14909.97 Da Isoelectric Point: 4.4693
>AT52374 WP_039800694.1 NZ_CP010415:c77246-76845 [Azotobacter chroococcum NCIMB 8003]
MSALIKQASEHWRYVAPLLTRPANEDDYDALVEALDELLVIVGDDEDHPLATLASHMGDLIEAYDEAQRPMPKVSGVEML
RYLMQEHGLTQADLPEIGVQSVVSELLSGKRRLNVRHIRALSERFGVPADVFF
MSALIKQASEHWRYVAPLLTRPANEDDYDALVEALDELLVIVGDDEDHPLATLASHMGDLIEAYDEAQRPMPKVSGVEML
RYLMQEHGLTQADLPEIGVQSVVSELLSGKRRLNVRHIRALSERFGVPADVFF
Download Length: 402 bp
>AT52374 NZ_CP010415:c77246-76845 [Azotobacter chroococcum NCIMB 8003]
ATGTCCGCGCTGATCAAACAAGCCAGCGAACACTGGCGCTATGTGGCCCCCTTGCTCACCAGGCCGGCCAACGAAGACGA
CTACGACGCCTTGGTCGAAGCCCTGGACGAATTGCTGGTTATCGTCGGCGACGACGAAGATCATCCCCTTGCCACCCTGG
CCTCGCACATGGGCGACCTGATCGAAGCCTACGATGAAGCCCAGCGTCCTATGCCGAAGGTGAGCGGCGTGGAGATGCTG
CGCTACCTGATGCAGGAGCATGGTCTGACTCAGGCCGATCTGCCGGAGATCGGCGTGCAGTCCGTCGTTTCCGAGCTGCT
CAGCGGCAAGCGCCGGCTCAATGTGCGCCATATCCGCGCGTTATCCGAGCGCTTTGGTGTACCGGCGGATGTTTTCTTTT
GA
ATGTCCGCGCTGATCAAACAAGCCAGCGAACACTGGCGCTATGTGGCCCCCTTGCTCACCAGGCCGGCCAACGAAGACGA
CTACGACGCCTTGGTCGAAGCCCTGGACGAATTGCTGGTTATCGTCGGCGACGACGAAGATCATCCCCTTGCCACCCTGG
CCTCGCACATGGGCGACCTGATCGAAGCCTACGATGAAGCCCAGCGTCCTATGCCGAAGGTGAGCGGCGTGGAGATGCTG
CGCTACCTGATGCAGGAGCATGGTCTGACTCAGGCCGATCTGCCGGAGATCGGCGTGCAGTCCGTCGTTTCCGAGCTGCT
CAGCGGCAAGCGCCGGCTCAATGTGCGCCATATCCGCGCGTTATCCGAGCGCTTTGGTGTACCGGCGGATGTTTTCTTTT
GA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A0C4WKM4 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A0C4WIL7 |