Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1174630..1175357 Replicon chromosome
Accession NZ_CP010384
Organism Enterobacter hormaechei subsp. xiangfangensis strain 34399

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3V067
Locus tag LI66_RS05505 Protein ID WP_022650547.1
Coordinates 1175046..1175357 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B6A245
Locus tag LI66_RS05500 Protein ID WP_039268734.1
Coordinates 1174630..1175049 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI66_RS05485 1171702..1172823 - 1122 WP_003858868.1 glutamate--cysteine ligase -
LI66_RS05490 1172929..1173819 + 891 WP_022650544.1 oxidoreductase -
LI66_RS05495 1173872..1174306 - 435 WP_022650545.1 hypothetical protein -
LI66_RS05500 1174630..1175049 - 420 WP_039268734.1 helix-turn-helix domain-containing protein Antitoxin
LI66_RS05505 1175046..1175357 - 312 WP_022650547.1 type II toxin-antitoxin system HigB family toxin Toxin
LI66_RS26085 1176050..1176469 + 420 WP_032645895.1 hypothetical protein -
LI66_RS05515 1176582..1177037 - 456 WP_022650549.1 hypothetical protein -
LI66_RS05520 1177320..1178201 - 882 WP_022650550.1 LysR family transcriptional regulator -
LI66_RS05525 1178307..1179086 + 780 WP_003858848.1 acetolactate decarboxylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Antitoxin HigA

(6-137)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12554.34 Da        Isoelectric Point: 9.9435

>T52302 WP_022650547.1 NZ_CP010384:c1175357-1175046 [Enterobacter hormaechei subsp. xiangfangensis]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Download         Length: 312 bp

>T52302 NZ_CP010384:c1175357-1175046 [Enterobacter hormaechei subsp. xiangfangensis]
ATGCACATAGTCTCAAGAGCGCCTTTCGACACAGCGGCCAGACATTATCCCAATCAAGCACAAGCCCTGGACGATCTTTA
CCGGGTACTAAAAAAGGAACACTACCCCTCACCAGACGAGATGCGAAAGCGCTTTCCCAGCCTGGACCGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATCTAAGAGTAATGTTTTTTGCTGATTTTGAGCGGGGAAAGTTC
TTCATCAAACACATCAGCACGCACGCAGAGTATGACAAGCTCACGGACTACTATCGGAGAACGAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15463.51 Da        Isoelectric Point: 4.6051

>AT52302 WP_039268734.1 NZ_CP010384:c1175049-1174630 [Enterobacter hormaechei subsp. xiangfangensis]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDSLALVEYLIEHDPDNPLVEMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp

>AT52302 NZ_CP010384:c1175049-1174630 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGAACTCCCTTTCCTGGGTTCAAGCCCGTCGCGGCA
AGACTACGAGGATTCGCTGGCGCTGGTGGAGTATCTTATCGAGCACGACCCGGATAACCCGCTGGTAGAGATGCTTACTG
CCAAAATCGATAAGTACGAAAACGAGTCCCCGGAGTTTGCAGAATTTAACGCGCGTATTGCCAGTATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTAATGGATCAGTACCAGCTCACTCAGAGCGACTTCGAGAACGAGATTGGTAAGAAGTCTCTGGT
AAGTCGGATCCTGAACGGACAGCGCACGTTAACGCTGGATCATATGCGGGCGCTGGCGAAGCGTTTTGGTTTGCCGGTGA
GCGCATTTGTGGGGAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3V067

Antitoxin

Source ID Structure
AlphaFold DB A0A8B6A245

References