Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2926226..2926886 Replicon chromosome
Accession NZ_CP010376
Organism Enterobacter hormaechei subsp. steigerwaltii strain 34977

Toxin (Protein)


Gene name higB Uniprot ID A0A2J0Q4X0
Locus tag LI62_RS14235 Protein ID WP_017383312.1
Coordinates 2926533..2926886 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0Q4Z7
Locus tag LI62_RS14230 Protein ID WP_017383313.1
Coordinates 2926226..2926528 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LI62_RS14215 2922401..2923725 + 1325 Protein_2756 SidA/IucD/PvdA family monooxygenase -
LI62_RS14220 2923752..2925941 + 2190 WP_023303946.1 TonB-dependent siderophore receptor -
LI62_RS14230 2926226..2926528 - 303 WP_017383313.1 helix-turn-helix transcriptional regulator Antitoxin
LI62_RS14235 2926533..2926886 - 354 WP_017383312.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LI62_RS14240 2927077..2928027 - 951 WP_017383311.1 HTH-type transcriptional regulator Cbl -
LI62_RS14245 2928124..2929041 - 918 WP_003859531.1 nitrogen assimilation transcriptional regulator NAC -
LI62_RS14255 2929573..2930505 - 933 WP_040117824.1 L,D-transpeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(18-89)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13537.37 Da        Isoelectric Point: 9.9538

>T52280 WP_017383312.1 NZ_CP010376:c2926886-2926533 [Enterobacter hormaechei subsp. steigerwaltii]
VWAIKTTDRFDDWFTSLNDSERASVLAALLVLRERGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREYTRWLNTLKERN

Download         Length: 354 bp

>T52280 NZ_CP010376:c2926886-2926533 [Enterobacter hormaechei subsp. steigerwaltii]
GTGTGGGCGATTAAAACTACGGACAGGTTTGACGACTGGTTCACCTCATTAAACGATAGCGAGAGAGCCAGCGTACTGGC
CGCGCTACTTGTTTTGCGGGAGAGGGGACCGGGTCTGTCCAGACCGTATGCCGATACGCTGAAAGGCTCCCGCCACAGCA
ACATGAAAGAGTTGCGTATCCAGAGTAAGGGTGATCCGCTGAGGGCATTTTTCGCTTTCGATCCGAACCGTACGGGTATT
GTACTGTGTGCGGGCAATAAGGTCGGTAATGAAAGGCGATTTTACGATGAGATGCTCCTCGTTGCGGATCGGGAATACAC
TCGCTGGCTGAACACATTAAAAGAGAGGAATTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11028.85 Da        Isoelectric Point: 6.4727

>AT52280 WP_017383313.1 NZ_CP010376:c2926528-2926226 [Enterobacter hormaechei subsp. steigerwaltii]
MGRTLEQLIADEKPEVVASAQAMATDILLNIHLAELREKVQKTQVDMARALGIKQPTVAVMEKAGRDIKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Download         Length: 303 bp

>AT52280 NZ_CP010376:c2926528-2926226 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGGCAGAACCCTGGAGCAGCTTATTGCAGATGAAAAACCTGAGGTTGTTGCCAGCGCACAGGCAATGGCAACGGATAT
TTTGCTCAATATCCATCTGGCCGAGTTACGTGAAAAAGTGCAAAAAACGCAGGTAGATATGGCCAGGGCGTTGGGTATTA
AACAACCTACTGTCGCCGTCATGGAAAAGGCAGGACGCGATATTAAGCTTTCTTCTTTGAAACGCTACGTTGAGGCCGCC
GGAGGGAAGCTACGGCTGGATGTAGAACTACCCGATGGTTCTCATTACGAGTTTGTGTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0Q4X0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0Q4Z7

References