Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 136814..137471 Replicon plasmid p6409-202.186kb
Accession NZ_CP010373
Organism Escherichia coli strain 6409

Toxin (Protein)


Gene name higB Uniprot ID U3PDC3
Locus tag RR31_RS25275 Protein ID WP_000270043.1
Coordinates 136814..137164 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag RR31_RS25280 Protein ID WP_000124640.1
Coordinates 137169..137471 (+) Length 101 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RR31_RS25235 132480..132857 + 378 WP_000656305.1 GNAT family N-acetyltransferase -
RR31_RS25240 133058..133717 - 660 WP_000412211.1 type A-1 chloramphenicol O-acetyltransferase -
RR31_RS25245 133996..134693 + 698 Protein_148 IS1 family transposase -
RR31_RS25250 134723..135193 - 471 WP_001281821.1 hypothetical protein -
RR31_RS28535 135186..135557 - 372 WP_000516916.1 hypothetical protein -
RR31_RS28540 135568..135762 - 195 WP_000343597.1 hypothetical protein -
RR31_RS25270 136103..136651 - 549 WP_001061574.1 transcriptional regulator -
RR31_RS25275 136814..137164 + 351 WP_000270043.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RR31_RS25280 137169..137471 + 303 WP_000124640.1 XRE family transcriptional regulator Antitoxin
RR31_RS25285 137498..137791 - 294 WP_001239997.1 chromosome segregation protein ParM -
RR31_RS25290 137879..138151 - 273 WP_001043047.1 HU family DNA-binding protein -
RR31_RS25295 138209..138736 - 528 WP_040110415.1 thermonuclease family protein -
RR31_RS25300 138753..138941 - 189 WP_001270411.1 hypothetical protein -
RR31_RS25305 138967..139824 - 858 WP_001167032.1 hypothetical protein -
RR31_RS25310 139811..140041 - 231 WP_000972663.1 hypothetical protein -
RR31_RS25315 140041..140559 - 519 WP_000210756.1 nitrite reductase -
RR31_RS25320 140556..141002 - 447 WP_000919345.1 Fe3+-siderophore ABC transporter permease -
RR31_RS25325 141002..141361 - 360 WP_000422768.1 hypothetical protein -
RR31_RS25330 141418..141846 - 429 WP_000591074.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 / aph(3'')-Ib / aph(6)-Id / aac(6')-Ian / tet(B) / sul1 / qacE / aadA10 / dfrA1 / catA1 htpB / htpB / htpB 1..193908 193908


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-106)

Antitoxin

(33-79)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 13328.21 Da        Isoelectric Point: 5.2421

>T52265 WP_000270043.1 NZ_CP010373:136814-137164 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Download         Length: 351 bp

>T52265 NZ_CP010373:136814-137164 [Escherichia coli]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11006.73 Da        Isoelectric Point: 7.2036

>AT52265 WP_000124640.1 NZ_CP010373:137169-137471 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Download         Length: 303 bp

>AT52265 NZ_CP010373:137169-137471 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7G8AFB9


Antitoxin

Source ID Structure

References