Detailed information of TA system TA052261

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 67896..68150
• Replicon: plasmid p6409-151.583kb
• Accession: NZ_CP010372
• Organism: Escherichia coli strain 6409

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: RR31_RS24000
• Protein ID: WP_015387399.1
• Coordinates: 67944..68150 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	67896..67957 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RR31_RS23960	63548..63760	+	213	WP_005012601.1	hypothetical protein	-
RR31_RS29265	64061..64150	-	90	Protein_75	IS1 family transposase	-
RR31_RS23970	64205..64882	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
RR31_RS23975	64882..65229	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
RR31_RS23980	65249..66820	+	1572	WP_023149734.1	IS66 family transposase	-
RR31_RS27585	66858..67474	-	617	Protein_79	IS1 family transposase	-
RR31_RS23995	67575..67757	+	183	WP_000968309.1	hypothetical protein	-
-	67896..67957	-	62	NuclAT_1	-	Antitoxin
-	67896..67957	-	62	NuclAT_1	-	Antitoxin
-	67896..67957	-	62	NuclAT_1	-	Antitoxin
-	67896..67957	-	62	NuclAT_1	-	Antitoxin
RR31_RS24000	67944..68150	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
RR31_RS24005	68434..68691	+	258	WP_000083833.1	replication regulatory protein RepA	-
RR31_RS27600	68927..69001	+	75	WP_032336874.1	RepA leader peptide Tap	-
RR31_RS24010	68994..69440	+	447	Protein_84	incFII family plasmid replication initiator RepA	-
RR31_RS27605	69386..69868	-	483	Protein_85	VENN motif pre-toxin domain-containing protein	-
RR31_RS24025	70761..71981	+	1221	WP_000410951.1	arginine deiminase	-
RR31_RS24030	71992..72903	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA1 / tet(B) / qacE / sul1 / blaTEM-1B / sitABCD	iucC / iucD / iutA / iucA / iucB	1..151583	151583

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T52261 WP_015387399.1 NZ_CP010372:67944-68150 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T52261 NZ_CP010372:67944-68150 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT52261 NZ_CP010372:c67957-67896 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin