Detailed information of TA system TA052258

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27814..28083
• Replicon: plasmid p6409-151.583kb
• Accession: NZ_CP010372
• Organism: Escherichia coli strain 6409

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: RR31_RS23760
• Protein ID: WP_001312861.1
• Coordinates: 27925..28083 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	27814..27879 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RR31_RS23720	23930..24495	+	566	Protein_26	class I SAM-dependent methyltransferase	-
RR31_RS23725	24521..24733	+	213	WP_001348622.1	hypothetical protein	-
RR31_RS23730	25143..25349	+	207	WP_000275856.1	hypothetical protein	-
RR31_RS23735	25375..25914	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
RR31_RS23740	25982..26215	+	234	WP_000005990.1	DUF905 family protein	-
RR31_RS27550	26243..26440	+	198	Protein_31	hypothetical protein	-
RR31_RS23745	26495..26929	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
RR31_RS23750	26926..27645	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
RR31_RS29260	27657..27845	-	189	WP_001299721.1	hypothetical protein	-
-	27657..27881	+	225	NuclAT_0	-	-
-	27657..27881	+	225	NuclAT_0	-	-
-	27657..27881	+	225	NuclAT_0	-	-
-	27657..27881	+	225	NuclAT_0	-	-
-	27814..27879	+	66	-	-	Antitoxin
RR31_RS23760	27925..28083	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
RR31_RS23775	29004..29291	+	288	WP_000107535.1	hypothetical protein	-
RR31_RS23780	29409..30230	+	822	WP_001234426.1	DUF945 domain-containing protein	-
RR31_RS23785	30527..31129	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
RR31_RS23790	31450..31833	+	384	WP_001151524.1	relaxosome protein TraM	-
RR31_RS23795	32020..32709	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA1 / tet(B) / qacE / sul1 / blaTEM-1B / sitABCD	iucC / iucD / iutA / iucA / iucB	1..151583	151583

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T52258 WP_001312861.1 NZ_CP010372:27925-28083 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T52258 NZ_CP010372:27925-28083 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT52258 NZ_CP010372:27814-27879 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin