Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2168579..2169081 Replicon chromosome
Accession NZ_CP010371
Organism Escherichia coli strain 6409

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RR31_RS10885 Protein ID WP_000767829.1
Coordinates 2168579..2168833 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RR31_RS10890 Protein ID WP_001259255.1
Coordinates 2168830..2169081 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RR31_RS10860 2163594..2163821 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
RR31_RS10865 2163835..2164893 - 1059 WP_000492321.1 transport protein YeeE -
RR31_RS10870 2165072..2166430 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RR31_RS28805 2166420..2166482 - 63 WP_010723108.1 membrane protein YoeI -
RR31_RS10875 2166697..2167626 - 930 WP_000803351.1 LysR family transcriptional regulator -
RR31_RS10880 2167672..2168496 - 825 WP_000754749.1 SDR family oxidoreductase -
RR31_RS10885 2168579..2168833 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RR31_RS10890 2168830..2169081 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RR31_RS26580 2169364..2169414 + 51 WP_001364200.1 his operon leader peptide -
RR31_RS10895 2169560..2170459 + 900 WP_000131777.1 ATP phosphoribosyltransferase -
RR31_RS10900 2170465..2171769 + 1305 WP_001614652.1 histidinol dehydrogenase -
RR31_RS10905 2171766..2172836 + 1071 WP_000108939.1 histidinol-phosphate transaminase -
RR31_RS10910 2172836..2173903 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T52246 WP_000767829.1 NZ_CP010371:c2168833-2168579 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T52246 NZ_CP010371:c2168833-2168579 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT52246 WP_001259255.1 NZ_CP010371:c2169081-2168830 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT52246 NZ_CP010371:c2169081-2168830 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References