Detailed information of TA system TA052230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 66686..67289
• Replicon: plasmid p6411-89.111kb
• Accession: NZ_CP010369
• Organism: Acinetobacter nosocomialis strain 6411

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: RR32_RS18495
• Protein ID: WP_000288004.1
• Coordinates: 66960..67289 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RR32_RS18490
• Protein ID: WP_001258993.1
• Coordinates: 66686..66973 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RR32_RS18465	63732..64202	+	471	WP_000395699.1	hypothetical protein	-
RR32_RS20040	64275..64421	+	147	WP_162097055.1	hypothetical protein	-
RR32_RS18470	64614..64850	+	237	WP_000152660.1	hypothetical protein	-
RR32_RS18475	65512..65967	+	456	WP_001136766.1	hypothetical protein	-
RR32_RS18480	66028..66489	+	462	WP_000378528.1	hypothetical protein	-
RR32_RS18485	66458..66649	+	192	WP_040118383.1	hypothetical protein	-
RR32_RS18490	66686..66973	-	288	WP_001258993.1	helix-turn-helix domain-containing protein	Antitoxin
RR32_RS18495	66960..67289	-	330	WP_000288004.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RR32_RS18500	67344..67613	-	270	WP_000453245.1	transcriptional regulator	-
RR32_RS18505	67820..68422	+	603	WP_000096286.1	hypothetical protein	-
RR32_RS18510	68479..68697	+	219	WP_000994925.1	hypothetical protein	-
RR32_RS18515	68790..68987	+	198	WP_025464206.1	hypothetical protein	-
RR32_RS18520	69118..70050	-	933	WP_000743263.1	IS5 family transposase	-
RR32_RS18525	70217..70402	+	186	WP_000755724.1	hypothetical protein	-
RR32_RS18530	70405..70917	+	513	WP_000464612.1	Fic family protein	-
RR32_RS18535	71159..71487	-	329	Protein_76	DUF4236 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89111	89111
	-	flank	IS/Tn	-	-	69118..70050	932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-90)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12571.69 Da
• Isoelectric Point: 8.8102

>T52230 WP_000288004.1 NZ_CP010369:c67289-66960 [Acinetobacter nosocomialis]
MYTIEYYSEDVQDEILTLPDGLLARYFNLTDRMVIYGANLGEPHTLPMSKGLFELRLKSQEGIARVMYCTLVGKRIVMLH
SFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 330 bp

>T52230 NZ_CP010369:c67289-66960 [Acinetobacter nosocomialis]
ATGTATACAATTGAATATTACAGCGAAGACGTTCAGGATGAGATTCTTACATTACCTGATGGTCTTTTGGCTCGATATTT
TAACTTAACTGACCGTATGGTGATTTACGGCGCAAACTTAGGAGAACCTCACACATTACCAATGAGTAAAGGACTATTTG
AGTTGCGCTTAAAATCTCAAGAAGGTATTGCTCGTGTTATGTATTGCACACTAGTCGGTAAACGCATTGTAATGCTCCAT
AGTTTTGTTAAGAAAACCCAAAAAACACCTAAGCAAGATCTAAACCTAGCACTTGACCGCATGAAAGAGGTGAAAAATGC
GAACACATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10565.30 Da
• Isoelectric Point: 10.1453

>AT52230 WP_001258993.1 NZ_CP010369:c66973-66686 [Acinetobacter nosocomialis]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTARRDAGLSQAQIAERMGTSVPAVSRLEKALITGKPSPSIATLQ
KYAAAIGKHVEVRFV

Nucleotide

• Length: 288 bp

>AT52230 NZ_CP010369:c66973-66686 [Acinetobacter nosocomialis]
ATGCGAACACATGAAGAAATGAAAGCACTTGCATTATCTCGTTCAGCAGTGCGTACCGAATACGAACGAATTGAACGTGA
AGAAATGCCCCTGCTCGATATGGTACTTACTGCACGTCGAGATGCAGGTCTATCCCAAGCGCAAATCGCGGAACGCATGG
GAACAAGTGTACCTGCCGTTTCACGGCTAGAAAAAGCATTGATCACAGGCAAACCATCACCATCTATTGCCACATTGCAA
AAATATGCTGCGGCAATTGGTAAACATGTTGAAGTGCGTTTTGTCTAA