Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/RelE-HTH |
| Location | 388861..389446 | Replicon | plasmid pXBB1-9 |
| Accession | NZ_CP010351 | ||
| Organism | Acinetobacter johnsonii XBB1 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | N9CJF7 |
| Locus tag | EHS42_RS19235 | Protein ID | WP_000897307.1 |
| Coordinates | 389126..389446 (-) | Length | 107 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | EHS42_RS19230 | Protein ID | WP_000369781.1 |
| Coordinates | 388861..389133 (-) | Length | 91 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EHS42_RS19195 | 384010..385054 | + | 1045 | Protein_382 | fimbrial protein | - |
| EHS42_RS19200 | 385236..385846 | + | 611 | Protein_383 | recombinase family protein | - |
| EHS42_RS19205 | 386036..386131 | - | 96 | Protein_384 | macrolide 2'-phosphotransferase Mph(E) | - |
| EHS42_RS19210 | 386132..386692 | - | 561 | Protein_385 | SulP family inorganic anion transporter | - |
| EHS42_RS19215 | 386942..387151 | - | 210 | WP_004976362.1 | hypothetical protein | - |
| EHS42_RS19225 | 387445..388770 | + | 1326 | WP_076611903.1 | ISNCY-like element ISAjo2 family transposase | - |
| EHS42_RS19230 | 388861..389133 | - | 273 | WP_000369781.1 | helix-turn-helix domain-containing protein | Antitoxin |
| EHS42_RS19235 | 389126..389446 | - | 321 | WP_000897307.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| EHS42_RS19240 | 389585..390004 | + | 420 | WP_001180106.1 | SMI1/KNR4 family protein | - |
| EHS42_RS19245 | 390084..390365 | - | 282 | WP_000985609.1 | putative addiction module antidote protein | - |
| EHS42_RS19250 | 390366..390668 | - | 303 | WP_000286964.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| EHS42_RS19255 | 390817..391002 | + | 186 | Protein_393 | hypothetical protein | - |
| EHS42_RS19260 | 391093..392225 | + | 1133 | Protein_394 | IS3-like element ISAba14 family transposase | - |
| EHS42_RS19265 | 392288..392632 | + | 345 | Protein_395 | hypothetical protein | - |
| EHS42_RS19270 | 392721..393035 | - | 315 | WP_004728120.1 | BrnA antitoxin family protein | - |
| EHS42_RS19275 | 393022..393309 | - | 288 | WP_000438825.1 | BrnT family toxin | - |
| EHS42_RS19280 | 393539..394144 | - | 606 | WP_000447788.1 | LysE family translocator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aph(3'')-Ib / aph(6)-Id / tet(Y) / ARR-3 / qacE / sul1 / blaPER-1 / aph(3')-VIa / aac(3)-IId / cmlB1 / mph(E) / msr(E) / blaOXA-58 | - | 1..398857 | 398857 | |
| - | inside | IScluster/Tn | blaOXA-58 | - | 387433..398757 | 11324 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 107 a.a. Molecular weight: 12187.95 Da Isoelectric Point: 7.1639
>T52216 WP_000897307.1 NZ_CP010351:c389446-389126 [Acinetobacter johnsonii XBB1]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG
Download Length: 321 bp
>T52216 NZ_CP010351:c389446-389126 [Acinetobacter johnsonii XBB1]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A
Antitoxin
Download Length: 91 a.a. Molecular weight: 10149.61 Da Isoelectric Point: 8.6582
>AT52216 WP_000369781.1 NZ_CP010351:c389133-388861 [Acinetobacter johnsonii XBB1]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI
Download Length: 273 bp
>AT52216 NZ_CP010351:c389133-388861 [Acinetobacter johnsonii XBB1]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|