Detailed information of TA system TA052214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 367822..368407
• Replicon: plasmid pXBB1-9
• Accession: NZ_CP010351
• Organism: Acinetobacter johnsonii XBB1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: EHS42_RS19110
• Protein ID: WP_000897307.1
• Coordinates: 368087..368407 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EHS42_RS19105
• Protein ID: WP_000369781.1
• Coordinates: 367822..368094 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHS42_RS19085	362895..363857	-	963	WP_033917576.1	IS30 family transposase	-
EHS42_RS19090	364113..364724	+	612	WP_058952606.1	recombinase family protein	-
EHS42_RS19095	364914..365798	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
EHS42_RS19100	365854..367329	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
EHS42_RS19105	367822..368094	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
EHS42_RS19110	368087..368407	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EHS42_RS19115	368531..368968	-	438	Protein_366	ISNCY family transposase	-
EHS42_RS19120	369110..369649	-	540	WP_049068995.1	plasmid replication DNA-binding protein	-
EHS42_RS19125	369717..370634	-	918	WP_049068997.1	replication initiation protein RepM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id / tet(Y) / ARR-3 / qacE / sul1 / blaPER-1 / aph(3')-VIa / aac(3)-IId / cmlB1 / mph(E) / msr(E) / blaOXA-58	-	1..398857	398857
	-	inside	IScluster/Tn	mph(E) / msr(E)	-	362895..368827	5932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T52214 WP_000897307.1 NZ_CP010351:c368407-368087 [Acinetobacter johnsonii XBB1]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T52214 NZ_CP010351:c368407-368087 [Acinetobacter johnsonii XBB1]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT52214 WP_000369781.1 NZ_CP010351:c368094-367822 [Acinetobacter johnsonii XBB1]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT52214 NZ_CP010351:c368094-367822 [Acinetobacter johnsonii XBB1]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3